低频脉冲电磁场影响超顺磁性氧化铁纳米颗粒标记BMSCs的增殖和成软骨分化  

LFPEMFs effects on the proliferation and chondrocyte-like differentiation of BMSCs labeled with SPIO

作　　者：方楚玲[1] 龚望球 黄婉静 周彬[1] 栗园 田京[2] 

机构地区：[1]南方医科大学第二临床医学院,广州510282 [2]南方医科大学珠江医院骨科,广州510282

出　　处：《中国临床解剖学杂志》2015年第6期662-666,共5页Chinese Journal of Clinical Anatomy

基　　金：广东省科技计划资助项目(2011B031800147);大学生创新实验项目基金(1212112013)

摘　　要：目的探讨外加低频脉冲电磁场(low frequency pulsed electromagnetic fields,LFPEMFs)对超顺磁性氧化铁纳米颗粒(superparamagnetic iron oxide nanoparticle,SPIO)标记的骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)增殖和成软骨分化的影响。方法体外培养BMSCs并进行SPIO标记。将SPIO标记的细胞分为4组并给予不同干预:LFPEMFs组,成软骨诱导组,LFPEMFs+成软骨诱导组,对照组。采用CCK8检测各组细胞的增殖情况,PCR及免疫荧光染色检测BMSCs成软骨分化水平。结果CCK8检测表明,LFPEMFs刺激促进细胞增殖;分化培养21 d时,LFPEMFs+成软骨诱导组细胞的Aggrecan和CollagenⅡ表达量明显高于对照组(P<0.05)。结论LFPEMFs刺激可以促进SPIO标记的BMSCs的增殖和成软骨分化。Objective To explore the effects of low frequency pulsed electromagnetic fields （LFPEMFs）on the proliferation and chondrocyte-like cells differentiation of BMSCs labeled with superpar-amagnetic iron oxide nanoparticle（SPIO）. Methods Labeled BMSCs were developed and divided into four groups, followed by different treatments：LFPEMFs group, chondrocyte-induced group, LFPEMFs and chondrocyte-induced group, and control group. The proliferation and chondrocyte-like differentiation of cells were measured accordingly, adopting CCK8, PCR or immunofluorecence staining, respectively. Results Compared with control group, the proliferation of BMSCs increased significantly in LFPEMFs stimulated cells. The expression level of Aggrecan and Collagen Ⅱ mRNA and protein in groups with LFPEMFs stimulation was significantly higher （P〈0.05）. Conclusions This experiment proves that LFPEMFs can promote the proliferation and chondrocyte-like cells differentiation of SPIO labeled BMSCs.

关 键 词：LFPEMFs BMSCS 增殖 AGGRECAN CollagenⅡ 

分 类 号：Q954.657[生物学—动物学]