郑州地区2013～2014年秋冬季腹泻婴幼儿轮状病毒VP7基因分型  

The analysis about VP7 genotypes of rotavirus in infantile diarrhea in Zhengzhou from 2013 to 2014

作　　者：王艳丽[1] 娄俊丽 杜远举 李春力[4] 程艳波[1] 

机构地区：[1]郑州市第一人民医院儿科,河南郑州450003 [2]汕头航空有限公司航卫室,广东汕头515041 [3]河南省出入境检验检疫局,河南郑州450000 [4]郑州人民医院儿科,河南郑州450000

出　　处：《中国现代医生》2015年第33期45-47,共3页China Modern Doctor

基　　金：河南省科学技术成果(9412015Y0379)

摘　　要：目的了解郑州地区秋冬季腹泻患儿轮状病毒（rotavirus,RV）G基因型。方法收集郑州市第一人民医院及郑州人民医院儿科门诊及住院部2013年9月-2014年3月患儿的粪便标本500份,采用胶体金法检测RV抗原,阳性标本进行基因分型。结果 500份标本中,202份RV抗原阳性,阳性率为40.4%;阳性标本进行基因分型,G血清型以G1型（68.8%）为主要流行型别,其次为G3型（17.8%）,G1和G3混合感染型（7.9%）,G9型（1.5%）。结论郑州地区A组RV以G1P8型为主,有混合感染型。Objective To study the G genotype changings of group A rotavirus in infantile diarrhea in Zhengzhou.Methods A total of 500 stool samples were collected from children who were in hospital and outpatients in the department of peadiatrics, the first people＇s hospital and the people＇s hospital of Zhengzhou from September 2013 to March2014. Gold assay（ GICA） kit was used to detect rotavirus genemic RNA. The positive samples were performed to i dentify rotavirus P and G genotypes. Results 202 samples were detected rotavirus from 500 samples,the positive rate was 40.4%. 202 positive samples were performed to identify genotypes. Serotyped G1（68.8%） were dominated the main prevalence,the second genotypes were type G3（17.8%）. The mixed infection of type G1 and G3（7.9%）, and G9（1.5%）was detected. Conclusion G1P8 is the main prevalent type in Zhengzhou. There are complicated samples.

关 键 词：轮状病毒 基因型 腹泻 婴幼儿 

分 类 号：R725.1[医药卫生—儿科]