烟草电压依赖阴离子通道NtVDAC1.1的电子克隆及生物信息学分析  

In silico Cloning and Bioinformatics Analysis of Voltage- Dependent Anion Channel Gene Nt VDAC1.1 from Nicotiana tabacum

作　　者：武兆云[1] 张小全[1] 杨铁钊[1] 王红[1] 

机构地区：[1]河南农业大学烟草学院,河南郑州450002

出　　处：《山东农业科学》2015年第11期5-11,共7页Shandong Agricultural Sciences

基　　金：中国烟草总公司特色优质烟叶开发重大专项(Ts-01-2011003)

摘　　要：为研究电压依赖阴离子通道（VDAC）基因在烟草根系分泌有机酸的作用,进而为了解该基因的功能和应用提供基础,以拟南芥At VDAC1（NM_110994）为搜索序列,应用电子克隆技术得到一条烟草VDAC基因全长c DNA,命名为Nt VDAC1.1。生物信息学分析表明该基因全长为1 018 bp,有着完整的开放阅读框,编码276个氨基酸。进化关系及同源性分析表明,Nt VDAC1.1与其他物种的VDAC有着较高的相似性,其中与茄科植物马铃薯三个膜孔蛋白有着很高的一致性（0.768-0.902）。功能分析显示,Nt VDAC1.1为膜孔蛋白,参与了阴离子的转运、阴离子转运调节和跨膜运输。二级和四级结构分析均表明,Nt VDAC1.1含有α-螺旋和β-折叠。氨基酸位点分析表明,该蛋白具有多种类型的修饰位点,其中的磷酸化位点说明该基因与信号转导有关,对阴离子通道活性具有调节作用。In order to investigate the function of voltage- dependent anion channel（ VDAC） gene in tobacco root secreting organic acid and provide basis for its research and application,a full- length c DNA sequence of VDAC gene,termed as Nt VDAC1. 1,was obtained using At VDAC1（ NM_110994） as search probe.Bioinformatics analyses showed that the full length of Nt VDAC1. 1 was 1 018 bp,which contained a complete open reading frame and encoded 276 amino acid residues. Homology and phylogenetic relationship analyses suggested that Nt VDAC1. 1 shared high similarity with VDAC of other plant species,and the identities with three porins from Solanaceae potato were 0. 768 -0. 902. Nt VDAC1. 1 belonged to porin,which took part in anion transport,regulation of anion transport and transmembrane transport. The prediction of secondary and tertiary structure indicated that Nt VDAC1. 1 had α- helixs and β- sheets. Amino acid site analysis suggested that Nt VDAC1. 1 possessed a number of types of modification sites. These phosphorylation sites indicated that the protein might take part in plant signal transduction and regulation of anion channel activity.

关 键 词：烟草 电压依赖阴离子通道 基因克隆 生物信息学分析 

分 类 号：S572[农业科学—烟草工业] Q781[农业科学—作物学]