HPLC法测定丹翘灌注液中丹参酮Ⅱ_A的含量  

Determination of Tanshinone Ⅱ_A in Danqiao perfusate by HPLC

作　　者：王东升[1] 苗小楼[1] 张世栋[1] 董书伟[1] 魏立琴[1] 那立冬 闫宝琪 严作廷[1] 

机构地区：[1]中国农业科学院兰州畜牧与兽药研究所农业部兽用药物创制重点实验室甘肃省中兽药工程技术研究中心甘肃省新兽药工程重点实验室,甘肃兰州730050

出　　处：《中兽医医药杂志》2015年第6期35-37,共3页Journal of Traditional Chinese Veterinary Medicine

基　　金："十二五"国家科技支撑计划项目(2012BAD12803);中国农业科学院科技创新工程

摘　　要：测定丹翘灌注液中丹参酮Ⅱ_A含量。采用高效液相色谱法,色谱柱:Kromasil:100-5 C_(18)(4.6 mm×250 mm,5μm);流动相:甲醇-水(75:25),流速:1.0 mL/min,柱温:30℃,检测波长:270 nm。结果:丹参酮Ⅱ_A在0.217 6-2.176 0μg范围內线性关系良好(r=0.9997),丹参酮Ⅱ_A的平均回收率为100.03%,RSD为0.57%。丹翘灌注液中丹参酮Ⅱ_A含量为0.9797 mg/mL。结果表明采用高效液相色谱法测定丹翘灌注液中丹参酮Ⅱ_A含量简便、快速、灵敏、准确,可用于该制剂中丹参酮Ⅱ_A的含量测定。To determine tanshinone Ⅱ_A in Danqiao perfusate,the method of high performance liquid chromatography was carried out with C_（18） column and a mixture of methanol and water（75：25） as the mobile phase.As spectrophotometer set at270 nm,flow rate was 1.0 mL/min.The number of theoretical plates of the column was not less than 2000,calculated with the reference to the peak of tanshinoneⅡ_A.The results showed that the linear range for the content of tanshinone Ⅱ_Awas 0.217 6 —2.176 0 μg,r=0.999 7,the average recovery rate of tanshinone Ⅱ_A was 100.03%and RSD was 0.57%,the content of tanshinone Ⅱ_A in Danqiao perfusate was 0.979 7 mg/mL.These indicated that the method for determination of tanshinone Ⅱ_Ain Danqiao perfusate with HPLC is convenient,rapid,sensitive,accurate and practical.

关 键 词：丹翘灌注液 丹参酮Ⅱ_A 高效液相色谱法 含量测定 

分 类 号：S853.7[农业科学—临床兽医学]