采用蒽酮法测定纤维素酶活力的最适条件  

Optimal conditions for cellulase activity measurement with anthrone method

作　　者：李静[1] 崔景东[1] 李维华[1] 

机构地区：[1]内蒙古工业大学轻工与纺织学院,呼和浩特010080

出　　处：《产业用纺织品》2015年第10期39-43,共5页Technical Textiles

摘　　要：研究蒽酮试剂比色法测定羧甲基纤维素酶活力的最适试验条件,如温度、p H值、酶促反应时间、波长等。试验表明,最佳测试条件为:温度50℃,最大吸收波长630 nm,羧甲基纤维素钠(CMC-Na)底物质量分数0.25%,酶促反应时间30 min,p H值5.2,原酶液稀释6倍,蒽酮用量3 m L。The conditions, such as temperature, pH value, enzymatic reaction time, wavelength and so on, for measuring carboxy methy cellose （ CMC ） enzyme activity were studied with anthrone colorimetric method. Experimental results show that the optimum test condition is 50 ℃, the maximum absorption wavelength is 630 nm, the substrate of sodium carboxy methyl cellulose （ CMC-Na） is 0. 25%, the enzymatic reaction time is 30 min, pH value is 5. 2, the original enzyme solution is diluted 6-fold, and anthrone amount is 3 mL.

关 键 词：纤维素酶活力 蒽酮试剂 葡萄糖 最佳测试条件 

分 类 号：TS190.2[轻工技术与工程—纺织化学与染整工程]