猪传染性胃肠炎病毒感染PK15细胞抗病毒相关因子转录变化分析  

Analysis of antiviral cytokines transcriptional profiles of PK15 cells cultures following infection with transmissible gastroenteritis virus

作　　者：胡晓亮[1,2] 刘家森[2] 田进[2] 姜骞[2] 郭东春[2] 曲娟娟[1] 曲连东[2,3] 

机构地区：[1]东北农业大学生命科学学院,哈尔滨150030 [2]中国农业科学院哈尔滨兽医研究所,兽医生物技术国家重点实验室,哈尔滨150001 [3]东北农业大学动物医学学院,哈尔滨150030

出　　处：《东北农业大学学报》2015年第11期22-27,共6页Journal of Northeast Agricultural University

基　　金：“十二五”农村领域国家科技计划课题项目(2012BAD46B01)

摘　　要：为研究猪传染性胃肠炎病毒感染与固有免疫之间关系,研究构建含有巴马猪IFN-α1,IFN-β和NF-κB启动子的报告基因载体,将转染上述报告基因的PK15细胞接种TGEV和灭活TGEV后发现,与灭活TGEV相比,TGEV能够诱导IFN-α1、IFN-β和NF-κB报告基因低水平表达;其次,利用荧光定量RT-PCR方法检测接种病毒0、2、6和10 h后PK15细胞中IFN-α1、IFN-β、IL6、TNF-α、ISG56和IRF7转录量,结果表明,TGEV对IL6转录没有影响,而诱导IFN-α1、IFN-β、TNF-α、ISG56和IRF7低水平转录,分别为对照组1.49、1.5、2.2、2.5和1.8倍。In order to survey the relationship between transmissible gastroenteritis virus infection and host innate immunity responses. Promoters of IFN- α1, IFN- β and NF- κB were constructed and then transfected into PK15 cells infected by TGEV and inactive TGEV. We found that TGEV could induce the weak expression of IFN-α1, IFN-β and NF-κB, compared with inactive TGEV. Furthermore, Real-timeq PCR was carried out to detect the transcript levels of cytokines（IFN-α1, IFN-β, IL6, TNF-α, IRF7 and ISG56） at 0,2,6 and 10 h. The results showed that the transcription levels of IFN-α1, IFN-β, TNF-α, ISG56 and IRF7 was1.49, 1.5, 2.2, 2.5 and 1.8 times with TGEV infection compared with inactive TGEV-HX, excepted IL6.

关 键 词：猪传染性胃肠炎 细胞因子 PK15细胞 转录时相 

分 类 号：S858.28[农业科学—临床兽医学]