冷刺激下AA肉鸡FBP1基因表达量的研究  

Study on the Expression of FBP1 Gene in AA Broilers under Cold Stimulating Environment

作　　者：刘火[1] 杜晓彤 闫辰[1] 包军[2] 李剑虹[1] 

机构地区：[1]东北农业大学生命科学学院,哈尔滨150030 [2]东北农业大学动物科学技术学院,哈尔滨150030

出　　处：《中国畜牧兽医》2015年第12期3146-3152,共7页China Animal Husbandry & Veterinary Medicine

基　　金：黑龙江省自然基金项目"鸡慢性应激模型的建立及应激相关基因的筛选"(C200932)

摘　　要：为了解冷刺激条件下果糖-1,6-二磷酸酶1(FBP1)基因在肉鸡组织中的表达情况,试验选择75只AA肉鸡随机分成了对照组(正常饲养温度)和4个处理组(比正常饲养温度低3℃),从雏鸡8日龄开始每天分别进行1、3、5和24h的冷刺激,在21日龄时结束冷刺激,并于22日龄屠宰测定FBP1基因在AA肉鸡各组织中的表达情况。结果表明,在冷刺激条件下,FBP1基因表达量存在组织特异性,在1h处理组中FBP1基因在肝脏中的表达量显著高于其他各组织(P<0.05),在3h处理组中FBP1基因在肺脏中表达量显著高于除肝脏外的其他各组织(P<0.05),在5h处理组中FBP1基因在肝脏中的表达量显著高于其他各组织(P<0.05),在24h处理组中FBP1基因在胸腺中的表达量明显高于其他各组织(P<0.05)。在相同组织不同处理组中,心脏FBP1基因的表达量除3h组明显降低外,其余处理组随冷刺激时间延长呈下降趋势,肝脏中FBP1基因表达量在5h组中显著高于其他各处理组(P<0.05),脾脏中FBP1基因的表达量除在1h组中明显降低外,其余各处理组随冷刺激时间延长呈下降趋势,肺脏中FBP1基因的表达量呈先上升后下降的趋势,肾脏中FBP1基因表达量除在24h组中明显降低外,其余各组没有明显变化,胸腺中24h组FBP1基因表达量显著高于其他处理组(P<0.05),且其他处理组与对照组差异不显著(P>0.05)。In order to study of FBP1 gene expression under cold stimulating environment,75 AA broilers as research object were selected and randomly assigned to 5groups,one of group was control group that raising in normal temperature,the remaining four groups were cold stimulation groups that raising temperature was 3 ℃lower than the control group.The time of cold stimulation in four groups were 1,3,5and 24 h,respectively.Cold stimulation started at 8days of age,ended at 21 days of age.Broilers were slaughtered at 22 days of age and investigated FBP1 gene expression in each group.The results showed that the expression of FBP1 gene existed tissue specificity under cold stimulating environment.The relative expression level of FBP1 gene in liver was significantly higher than the other tissues in 1hgroup（P〈0.05）;The relative expression level of FBP1 gene in lung were significantly higher than the other tissues except of liver in 3h group except for liver（P〈0.05）;The relative expression level of FBP1 gene in liver were significantly higher than the other tissues in 5hgroup（P〈0.05）;The relative expression level of FBP1 gene in thymus were significantly higher than the other tissues in 24hgroup（P〈0.05）.FBP1 gene expression inside the heart was on the decline in the same organization different treat-ment groups as the rime of cold stimulating was increasing except for 3hgroup.FBP1 gene expression of liver in 5hgroup was significantly higher than other FBP1 gene expression in each treatment group.FBP1 gene expression of spleen was significant decreased in 1hgroup,the other group extend a downward trend with the extension of cold stimulation time.FBP1 gene expression in lung was downward trend after rising first.FBP1 gene expression in kidney was significantly decreased in 24 hgroup,and the other groups had no obvious change.FBP1 gene expression of thymus in 24 hgroup was significantly higher than the other groups（P〈0.05）,and there was no significant difference between control group and other four ter

关 键 词：AA肉鸡 冷刺激 FBP1基因 表达 

分 类 号：Q786[生物学—分子生物学]