肉鸡日粮中1α-羟基维生素D_3与25-羟基维生素D_3生物学效价比较研究  

Study on Relative Bioavailability of 1α-Hydroxycholecalciferol to 25-Hydroxycholecalciferol in Broiler Chickens

作　　者：韩进诚[1] 王建国[1,2] 陈冠华[1,2] 张金龙[1,2] 瞿红侠[1] 张进良[1] 席丽[1] 闫永峰[1] 

机构地区：[1]商丘师范学院生命科学学院,商丘476000 [2]河南农业大学牧医工程学院,郑州450002

出　　处：《中国畜牧兽医》2015年第12期3246-3252,共7页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金项目(31101732);河南省创新型科技人才队伍建设工程项目

摘　　要：本试验比较了肉鸡日粮中1α-羟基维生素D_3（1α-OH-D_3）与25-羟基维生素D_3（25-OH-D_3）生物学效价。将300只1日龄罗斯308肉鸡公雏随机分为6个处理,每个处理5个重复,每个重复10只鸡。设计6种日粮,即在相同的基础日粮中分别添加2.5、5.0和10.0μg/kg 25-OH-D_3和1.25、2.5和5.0μg/kg 1α-OH-D_3,斜率比法测定1α-OH-D_3与25-OH-D_3的相对生物学效价。结果显示,日粮中添加1α-OH-D_3和25-OH-D_3可改善肉鸡平均体增重、料重比及血浆磷浓度,显著增加胫骨和股骨重量、长度和灰分重量（P〈0.05）,而对骨骼钙和磷含量无显著影响（P〉0.05）。以1~42日龄肉鸡平均体增重、料重比和血浆磷浓度评价,1α-OH-D_3生物学效价分别是25-OH-D_3的2.16、2.56和2.77倍;以42日龄肉鸡胫骨重量、长度、宽度和灰分重量评价,1α-OH-D_3生物学效价是25-OH-D_3的1.80、1.88、2.42和2.04倍;以42日龄肉鸡股骨重量、长度和灰分重量评价,1α-OH-D_3生物学效价是25-OH-D_3的1.89、1.98和2.18倍。试验表明,以1~42日龄肉鸡生长性能和骨骼矿化指标评价,1α-OH-D_3生物学效价约为25-OH-D_3的2.17倍。The study was conducted to determine the relative bioavailability（RBV）of 1α-hydroxycholecalciferol（1α-OH-D_3）to 25-hydroxycholecalciferol（25-OH-D_3）in Ross 308 broiler chickens from 1to 42 days of age.300one-day-old Ross 308 broilers were randomly assigned into6 groups with 5replicates per group and 10 chicks per replicate.The six groups were fed with basal diets in which 2.5,5.0,10.0μg/kg 25-OH-D_3 and 1.25,2.5,5.0μg/kg 1α-OH-D_3 were added,respectively.The RBV of 1α-OH-D_3 to 25-OH-D_3 was determined by the slope ratio method.The results showed that average body weight gain（ABWG）,feed to gain ratio（F/G）,plasma phosphorus（P）were improved with the 1α-OH-D_3 and 25-OH-D_3 addition and the tibia and femur weight,length,and ash weight were linearly increased with the levels of 1α-OH-D_3 or 25-OH-D_3increasing（P〈0.05）.Using ABWG,F/G,and plasma P as the criteria,RBV of 1α-OH-D_3to25-OH-D_3 were 2.16,2.56,and 2.77.Using tibia weight,length,width,and ash weight as the criteria,RBV of 1α-OH-D_3 to 25-OH-D_3 were 1.80,1.88,2.42,and 2.04.Using femur weight,length,and ash weight as the criteria,RBV of 1α-OH-D_3 to 25-OH-D_3 were 1.89,1.98,and 2.18.These results indicated that the activity of 1α-OH-D_3 was approximately 2.17 times higher than that of 25-OH-D_3 in promoting growth performance and bone mineralization in broiler chickens from 1to 42 days of age.

关 键 词：1α-羟基维生素D3 25-羟基维生素D3 生物学效价 生长性能 骨骼矿化 肉鸡 

分 类 号：S831.5[农业科学—畜牧学]