某肉鸡场鸡源大肠杆菌耐药性跟踪检测与分析  

Tracking Detection and Analysis of Drug Resistance of Chicken-derived Escherichiacoliin a Broiler Farm

作　　者：王影[1] 李欣南 韩镌竹 刘英姿[3] 王宏军[3] 高慎阳[3] 吴思思[4] 张利勃[3] 周铁忠[3] 

机构地区：[1]吉林农业大学动物科学技术学院,长春130118 [2]辽宁省兽药饲料畜产品质量安全检测中心,沈阳110016 [3]辽宁医学院畜牧兽医学院,锦州121001 [4]爱丁堡大学

出　　处：《中国畜牧兽医》2015年第12期3329-3337,共9页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金(31201951);辽宁省兽药饲料畜产品质量安全检测中心合作项目:辽宁省鸡源大肠杆菌耐药性传播机制研究(20150101);辽宁省科技厅自然科学基金项目:辽宁肉鸡产业链中大肠杆菌耐药性及传递的研究(2015010784-301);辽宁省科技厅自然科学基金项目(2013022047;2013022043)

摘　　要：2012~2014年从辽宁某肉鸡养殖场采集300份鸡泄殖腔拭子,分离获得152株鸡源大肠杆菌。采用微量稀释法对152株鸡源大肠杆菌进行了14种兽用和16种人用抗菌药的药物敏感性测定,并参照CLSI肠杆菌判定标准进行判定。结果显示,鸡源大肠杆菌对兽用阿莫西林、氨苄西林、恩诺沙星、复方新诺明、磺胺异噁唑、四环素和人用哌拉西林、替卡西林、环丙沙星均产生明显耐药性,耐药率较高。对所分离的鸡源大肠杆菌耐药性检测结果与EUCAST发布的野生型菌株MIC值分布进行比较分析,耐药结果基本一致,但通过MIC值分布比较,结果显示出分离菌株的大观霉素、氨苄西林、恩诺沙星、复方新诺明、头孢噻吩、头孢呋辛、环丙沙星和妥布霉素药物的MIC值发生了明显右移,表明该肉鸡场大肠杆菌对上述抗菌药的耐药强度呈现逐渐增强的趋势。在养殖过程中应采取科学饲养和严格的生物安全措施防控疾病,科学合理使用抗菌药物,控制肉鸡场细菌耐药性的产生和传播。From 2012 to 2014,300 chicken cloacal swab samples were collected from a broiler farm in Liaoning province,152 strains of chicken-derived Escherichiacoliwere isolated and identified.The 152 strains of chicken-derived Escherichia coli were detected on 14 kinds of veterinary antimicrobial drugs and 16 kinds of antimicrobial drugs for human by using trace dilution method,and reference to CLSI enterobacter decision criteria to determine.The results showed that chicken-derived Escherichia coli had obvious resistance to veterinary antimicrobial drugs（amoxicillin,ampicillin,enrofloxacin,trimethoprim-sulfamethoxazole,sulfafurazole,tetracycline）and antimicrobial drugs for human（piperacillin,ticarcillin,ciprofloxacin）,the resistance rates were high.The resistance test results of isolated chicken-derived Escherichia coli comparing with the MIC value distribution of EUCAST wild type strains were same,but through comparing MIC value distribution,the results showed the isolates of spectinomycin,ampicillin,enrofloxacin,trimethoprim-sulfame-thoxazole,cefalotin,cefuroxime,ciprofloxacin and tobramycin drugs MIC value moved to the right obviously,indicated that the antibiotic resistant strength of Escherichia coli increased gradually.The process of breeding and strict biosecurity measures should be taken scientifically to control diseases,and the antibacterial agents should be applied reasonably to control broiler farm production and propagation of bacterial drug resistance.

关 键 词：肉鸡场 大肠杆菌 跟踪检测 耐药性分析 

分 类 号：S852.612[农业科学—基础兽医学]