甘木通总黄酮保护H_2O_2诱导的PC12细胞损伤  

Neuroprotective effects of total flavones from Clematis filamentosa against H_2O_2-induced injury to PC12 cells

作　　者：黄海潮[1] 聂阳[1] 张小红[1] 朱庆玲[1] 巫玮[1] 周捷[2] 

机构地区：[1]广东食品药品职业学院实验实训中心,广东广州510520 [2]中山大学肿瘤防治中心药学部,广东广州510060

出　　处：《中成药》2015年第12期2585-2589,共5页Chinese Traditional Patent Medicine

基　　金：广东省中医药局中医药强省项目(20141197);广东省医学科学技术研究基金项目(B2013071);广东食品药品职业学院自然科学项目(2012YZ007)

摘　　要：目的探讨甘木通(Clematis filamentosa)总黄酮(TFC)对H_2O_2引起的大鼠肾上腺嗜铬细胞瘤细胞(PC12细胞)损伤的保护作用。方法用H_2O_2损伤PC12细胞建立神经元氧化应激损伤模型,噻唑蓝(MTT)法检测细胞存活率,观察甘木通总黄酮对神经元损伤的作用;形态学以及Hoechst 33258染色观察TFC对细胞形态的影响;生化法测定超氧化物歧化酶(SOD)活性以及丙二醛(MDA)的量。结果与200μmol/L H_2O_2诱导的模型组对比,中(10μg/m L)、高(100μg/m L)剂量甘木通总黄酮预处理细胞形态好于模型组,细胞活性明显增强(P<0.05),细胞内的SOD活性增强(P<0.05),MDA水平减低(P<0.05)。结论甘木通总黄酮对H_2O_2引起的神经损伤有保护作用。AIM To study neuroprotective effects of total flavones from Clematis filamentosa（ TFC） on H2O2-induced injury to rat pheochromocytoma cells（ PC12 cells）. METHODS The viability of PC12 cells injured by H2O2 was determined by MTT assay. The cellular morphology was observed by Hoechst 33258 staining to evaluate protective effects of TFC on the injured cells. The SOD viability and MDA levels were detected by biochemical methods. RESULTS Compared with the model group,cell viability and SOD activity of TFC pretreated groups（ 10 μg /m L,100 μg /m L） were higher（ P〈0. 05） and the MDA level was lower（ P〈0. 05） obviously.CONCLUSION TFC shows neuroprotective effects on PC12 cells injured by H2O2.

关 键 词：甘木通 总黄酮 PC12细胞 SOD MDA 

分 类 号：R285.5[医药卫生—中药学]