利用叶片真空侵染法表达外源蛋白GFP  

Transient Expression of Foreign Gene GFP by Leaf-vacuum Agronoculation

作　　者：金太成[1] 孟大伟[1] 刘璐[1] 廖艺红 张爽[1] 罗佳尧 杨丽萍[1] 

机构地区：[1]吉林师范大学生命科学学院,四平136000

出　　处：《吉林农业科技学院学报》2015年第4期40-42,共3页Journal of Jilin Agricultural Science and Technology University

基　　金：国家青年科学基金项目(31301043);吉林省科技发展计划青年科研基金项目(20130522182JH);吉林省教育厅"十二五"科学技术研究项目(吉教科合字[2015]第231号)

摘　　要：植物瞬时表达系统已经成为近些年来国内外的研究热点之一,该表达系统主要的侵染技术为叶片注射法和真空侵染法。本研究成功构建了植物瞬时表达载体p35S-30B-GFP,并利用叶片真空侵染技术在烟草中表达外源基因GFP的研究,RT-PCR检测结果说明我们成功地表达了外源基因GFP。研究中对植物瞬时表达体系进行了优化试验,为植物中进行外源蛋白的快速表达奠定了研究基础。The transient expression system as plant bioreactor has been extensive researched recently. There were two main agronoculation methods, including leaf-infection and leaf-vacuum infiltration. We have constructed successfully transient expression vector p35S-30B-GFP derived from TMV virus, and expressed foreign protein GFP by RT-PCR biotechnology. Here, the transient expression system has been optimized and provided study base for expression of the foreign proteins in plants by means of transient expression system.

关 键 词：烟草 瞬时表达 烟草花叶病毒 绿色荧光蛋白 

分 类 号：Q943.2[生物学—植物学]