贵州部分柑橘地方品种遗传背景的SSR分析  

Genetic Background Analysis of Several Guizhou Local Citrus Varieties Based on SSR Markers

作　　者：雷天刚[1,2] 何永睿[1,2] 彭爱红[1,2] 许兰珍[1,2] 邹修平[1,2] 姚利晓[1,2] 李金强[3] 陈善春[1,2] 

机构地区：[1]西南大学柑桔研究所,重庆400712 [2]国家柑桔品种改良中心,重庆400712 [3]贵州省果树科学研究所,贵阳550006

出　　处：《西南大学学报（自然科学版）》2015年第11期30-35,共6页Journal of Southwest University(Natural Science Edition)

基　　金：科技部"863"项目(2011AA100205);国家自然科学基金(31272150);科技基础性工作专项(2012FY110200);中央高校基本业务费专项(XDJK2014C028;XDJK2014A018);重庆市应用开发项目基金(cstc2013yykfA80002)

摘　　要：利用SSR分子标记技术对5个贵州柑橘地方品种的遗传背景进行分析．结果表明，24对SSR引物在5个地方品种和参与D N A指纹比对的14个栽培品种中共检测到92个等位基因，每个位点的等位基因数变幅为2～7个，平均为3.8个；多态性信息含量（PIC）变化范围在0.36～0.78之间，平均为0.49．经过DNA指纹比对，发现其中5对引物扩增的8条特征谱带可作为地方品种鉴别的参考性标记．利用NTSYS软件进行相似性系数计算，采用 UP‐GM A法聚类，结果显示，相似性系数为0.80，可将5个地方品种划分在不同的亚组中；地方品种米柑和牛肉红金橘均单独成组，其遗传背景独特；土柑与皱皮柑的亲缘关系较近；大果型冰糖橙与冰糖橙的遗传背景相同；红香柚与强德勒柚的遗传背景基本一致．The genetic background of 5 local citrus varieties originating from Guizhou Province was ana‐lyzed by SSR marker technology .A total of 92 alleles amplified from 24 SSR primer pairs were detected in the 5 local varieties and other 14 citrus cultivars used for comparison of DNA fingerprinting .The number of alleles per SSR marker ranged from 2 to 7 with an average of 3.8 ,and the allelic polymorphism infor‐mation content （PIC） values varied from 0.36 to 0.78 with an average of 0.49 .DNA fingerprinting showed that 8 characteristic bands were amplified using 5 of those SSR primer pairs ,and they could be used as ref‐erence markers for the identification of those local varieties .The similarity coefficient was calculated using the NTSYS software ,and UPGMA cluster analysis showed that the 5 local varieties could be divided into 5 different subgroups at the genetic similarity coefficient of 0.80 .Of the five local varieties ,Migan and Ni‐urouhongjinju were grouped into different subclusters and each of them demonstrated a unique genotype . Tugan was closely related to Zoupigan .Large‐type Bingtang orange and Bingtang orange had the same ge‐netic background .The genetic background of Hongxiang pummelo and Chandler were almost exactly the same .T he available data herein provided a reference to the conservation and utilization of those accessions .

关 键 词：柑橘 地方品种 遗传背景 SSR标记 

分 类 号：S666[农业科学—果树学]