从Wnt/β-catenin信号通路探讨透骨消痛胶囊对关节软骨的保护作用  

To Investigate the Protective Effect of Tougu Xiaotong Jiaonang(透骨消痛胶囊) on Articular Cartilage from Wnt/β-catenin Signaling Pathway

作　　者：吴追乐[1] 曾建伟[2] 陈星强[2] 陈春蓉[2] 吴广文[2] 庄志强[3] 林如辉[2] 尤玉莹 郑春松[2] 

机构地区：[1]福建中医药大学针灸学院,福建福州350122 [2]福建中医药大学中西医结合研究院,福建福州350122 [3]福建中医药大学附属漳州市中医院,福建漳州363030

出　　处：《风湿病与关节炎》2015年第12期5-7,31,共4页Rheumatism and Arthritis

基　　金：国家自然科学基金资助项目(81202713)

摘　　要：目的:从Wnt/β-catenin信号通路观察透骨消痛胶囊对大鼠膝骨关节炎关节软骨的保护作用。方法:60只健康清洁级2月龄雄性SD大鼠,随机分为正常对照组、模型对照组和透骨消痛胶囊组,每组20只。模型对照组和透骨消痛胶囊组采用质4%木瓜蛋白酶双膝关节腔注射,成功复制骨关节炎模型后,透骨消痛胶囊组给予透骨消痛胶囊287.5 mg·kg^(-1),正常对照组和模型对照组给予相同剂量的生理盐水。给药2个月后,采用Western blot法观察各组关节软骨中含Ⅰ型血小板结合蛋白基序的解聚蛋白样金属蛋白酶-5(ADAMTS-5)、Wnt-4、β-catenin和基质金属蛋白酶-13(MMP-13)的表达。结果:与正常对照组比较,模型对照组ADAMTS-5、Wnt 4、β-catenin和MMP-13表达升高(P<0.05);与模型对照组比较,透骨消痛胶囊组ADAMTS-5、Wnt 4、β-catenin和MMP-13表达降低(P<0.05)。结论:透骨消痛胶囊对关节软骨的保护作用与其降低Wnt/β-catenin信号通路表达相关。Objective：To investigate the protective effect of Tougu Xiaotong Jiaonang（透骨消痛胶囊）on knee articular cartilage of rats from Wnt/β-catenin signaling pathway.Methods：Sixty healthy twomonth male SD rats were randomly divided into a normal control group,a model control group and a Tougu Xiaotong Jiaonang group（TXJ group）,20 rats in each.The model control group and the TXJ group were given double knee joint cavity injection with 4% papain.After the osteoarthritis models were successfully established,the TXJ group was given Tougu Xiaotong Jiaonang 287.5 mg·kg-1,while the normal control group and the model control group were given the same dose of physiological saline.After 2 months of administration,the Western blot method was used to observe the expressions of ADAMTS-5,Wnt-4,β-catenin and MMP-13 in articular cartilage of three groups.Results：Compared with the normal control group,the expressions of ADAMTS-5,Wnt-4,β-catenin and MMP-13 in the model control group increased（P 〈 0.05）,while compared with the model group,the expressions of ADAMTS-5,Wnt-4,β-catenin and MMP-13 in the TXJ group decreased（P 〈 0.05）.Conclusion：The protective effect of Tougu Xiaotong Jiaonang on articular cartilage is correlated with the reduction of the Wnt/β-catenin signaling pathway expression.

关 键 词：骨关节炎 膝 透骨消痛胶囊 WNT/Β-CATENIN信号通路 体内实验 大鼠 

分 类 号：R285.5[医药卫生—中药学]