单独感染HCV与HCV/HIV共感染患者外周血T淋巴细胞分化分析  

Analysis on the differentiation of peripheral T lymphocytes in mono-infected HCV and co-infected HCV/HIV patients

作　　者：覃岭[1] 赵艳[2] 孙焕芹[1] 刘宁[1] 江娜[1] 代艳超[1] 李昂[1] 王子康[1] 张永宏[1] 

机构地区：[1]首都医科大学附属北京佑安医院生物医学信息中心,100069 [2]首都医科大学附属北京佑安医院临床检验中心,100069

出　　处：《北京医学》2015年第12期1142-1145,共4页Beijing Medical Journal

基　　金：国家自然科学基金(81271842);"十二五"国家科技重大专项(2013ZX10001004-001-002);北京市自然科学基金(7132098);北京市科技计划(D131100005313004;D131100005313005)

摘　　要：目的探讨单独感染HCV与HCV/HIV共感染患者外周血T淋巴细胞分化情况。方法采集单独感染HCV患者(12例)、HCV/HIV共感染患者(27例)以及正常人群(13例)的外周静脉血,检测ALT、AST等生化指标;提取外周血单个核细胞(PBMC),通过流式细胞检测技术检测CD4+T和CD8+T细胞比例。结果 HCV/HIV共感染患者ALT水平[(53.08±9.78)U/L]显著高于单独感染HCV患者[(26.22±4.25)U/L],差异有统计学意义(P=0.0064)。处于中期分化状态的CD4+T细胞,在正常人群中所占比例为(35.13±3.24)%,显著高于单独感染HCV患者[(9.01±3.70)%]和HCV/HIV共感染患者[(8.10±2.14)%],差异有统计学意义(P<0.001)。正常人群、单独感染HCV患者与HCV/HIV共感染患者晚期分化的CD4+T细胞所占比例分别为(2.52±2.54)%、(14.30±9.85)%及(15.23±11.58)%,正常人群晚期分化的CD4+T细胞比例显著高于单感染HCV与HCV/HIV共感染患者的比例,差异有统计学意义(P<0.05),单独感染HCV与HCV/HIV共感染患者之间无差异。正常人群处于早期分化和中期分化的CD8+T细胞的比例分别为(42.73±2.08)%、(36.17±2.85)%,均显著高于单独感染HCV患者同期水平[(28.27±4.36)%、(13.15±4.23)%]及HCV/HIV共感染患者[(18.02±2.01)%、(16.60±3.06)%],差异均有统计学意义(P<0.05);单独感染HCV患者的早期分化CD8+T细胞比例也显著高于HCV/HIV共感染患者,差异有统计学意义(P<0.05)。而处于晚期分化的CD8+T细胞,正常人群所占比例为(12.02±1.54)%,显著低于单独感染HCV患者[(49.30±3.85)%]及HCV/HIV共感染患者[(54.23±2.58)%],差异有统计学意义(P<0.05)。结论单独感染HCV及HCV/HIV共感染影响T淋巴细胞的分化状态;HCV/HIV共感染影响处于早期分化的CD8+T细胞比例。Objective To explore the effect of mono-infected HCV and co-infected HCV/HIV patients on the differentiation of peripheral blood T lymphocytes. Methods Peripheral blood of HCV patients（12 cases）, HCV/HIV patients（27 cases）and healthy people（13 cases）were tested for the biochemical marker by chemistry analyzer, and the CD4＋T cells and CD8＋T cells were analyzed by FACS in PBMC. Results ALT level of co- infected HCV/HIV patients was（53.08±9.78）U/L, which was significantly higher than in the HCV group[（26.22±4.25）U/L, P = 0.0064]. The percentage of intermediate CD4＋T cells was（9.01±3.70）% in the HCV group,（8.10±2.14）% in the HCV/HIV group, which was significantly lower than in the healthy group[（35.13 ± 3.24）%, P〈 0.001]. The percentage of late CD4＋T cells was（2.52 ±2.54）%,（14.30±9.85）%,（15.23±11.58）% respectively in the healthy group, HCV group and HCV/HIV group. The percentage of CD4＋T cells in the healthy group was significantly lower than in the other two groups（P〈 0.05）, but there was no significant difference between HCV and HCV/HIV group. The percentage of early- stage CD8＋T cell in the healthy group was（42.73±2.08）%, which was significantly higher than in the HCV group [（28.27±4.36）%] and HCV/HIV group[（18.02±2.01）%], P all0.05. The percentage of early CD8＋T cells in HCV group was higher than in the HCV/HIV group（P〈 0.05）. The percentage of intermediate CD8＋T cell was（36.17 ± 2.85）%, which was significantly higher than in the HCV group [（13.15±4.23）%] and HCV/HIV group [（16.60±3.06）%], P〈 0.05. While the percentage of late CD8＋T cell in the healthy group was（12.02±1.54）%, which was significantly lower than in the HCV group [（49.30±3.85）%] and HCV/HIV group[（54.23±2.58）%], P〈 0.05. Conclusion Mono-infected HCV and（or） HCV/HIV co-infection do affect on the differentiation of T lymphocytes. HCV/HIV co-infection can affect on the early CD8＋T cells.

关 键 词：丙型肝炎病毒 人类免疫缺陷病毒 单独感染 共感染 淋巴细胞亚群 

分 类 号：R512.91[医药卫生—内科学] R512.63[医药卫生—临床医学]