利用Imaging-PAM研究小球藻和杜氏盐藻对石油胁迫的响应  

Using the Imaging-PAM to study the response of Chlorellasp.and Dunaliella salina on the oil stress

作　　者：李铁松[1] 王彤[1] 佟少明[1] 时颖[1] 王旭浩[1] 宁淑香[1] 

机构地区：[1]辽宁师范大学生命科学学院,辽宁大连116081

出　　处：《辽宁师范大学学报（自然科学版）》2015年第4期517-521,共5页Journal of Liaoning Normal University:Natural Science Edition

基　　金：辽宁师范大学博士启动基金项目;辽宁师范大学本科生科研训练项目

摘　　要：利用近岸海水大量培养微藻制备微藻燃料已成为新的研究热点,而近海海域常受到石油污染,因而研究微藻光合系统受石油胁迫的影响是确定藻种培养可行性的重要指标.单细胞藻悬液在暗处理后经激发,可检测到叶绿素的荧光信号.利用ImagingPAM来研究小球藻和杜氏盐藻的光合系统对石油胁迫的响应,通过分析2种微藻的初始荧光产量(F0)和充分暗适应后光合系统Ⅱ的最大光化学效率(Fv/Fm)得出小球藻自身的光合能力强于盐藻;通过分析非调节性能量耗散的量子产额Y(NO)、非光化学淬灭系数(NPQ)和光化学能量转化的有效量子产量[Y(Ⅱ)]得出小球藻在石油污染环境下光合作用的稳定性优于盐藻.因此,可以得出小球藻的光合系统受石油胁迫影响较小,较盐藻更适宜利用石油污染的海水进行培养.Using coastal seawater to culture a large scale of microalgae prepared as microalgae fuel has become a new hot topic.Because coastal waters are generally subject to oil pollution,studying the response of photosynthetic system of microalgae by oil stress is an important indicator to determine the feasibility of cultivation.The unicellular suspension of microalgae can be excited by appropriate light after dark treatment,and it can be used to detect the fluorescence signal of chlorophyll by fluorometer.In this paper,the Imaging-PAM is used to study the response of photosynthetic system of Chlorellasp.and Dunaliella salina on the oil stress.By the analysis of the minimum fluorescence（F0）and the maximal efficiency of PSⅡin a dark-adapted state（Fv/Fm）of two microalgae,we can draw that the Chlorellasp.own photosynthetic ability is better than Dunaliella salina;by the analysis of non-regulatory energy dissipation in the quantum yield Y（NO）,the nonphotochemical quenching parameter（NPQ）and the effective quantum yield of PSⅡ [Y（Ⅱ）]of the two microalgae,we can draw that photosynthesis stability under the oil pollution of Chlorellasp.is better than Dunaliella salina.Therefore,we conclude that the oil stress has less effect for the photosynthesis system of Chlorella sp.,and the Chlorella sp.is more appropriate than the Dunaliella salina cultured in oil-pollution waters.

关 键 词：Imaging-PAM 小球藻 杜氏盐藻 石油污染 叶绿素荧光 

分 类 号：Q89[生物学]