利用籼粳交RILs群体的水稻粒形QTLs定位  

Mapping of the QTLs for Rice Grain Shape Using Recombinant Inbred Lines of Indica-Japonica Hybridization in Rice

作　　者：刘丹[1,2] 王嘉宇[1] 柴永山[2] 孙玉友[2] 孙国宏[2] 冯章丽[2] 陈温福[1] 

机构地区：[1]沈阳农业大学水稻研究所/农业部东北水稻生物学与遗传育种重点实验室/辽宁省北方粳稻遗传育种重点实验室,沈阳110161 [2]黑龙江省农业科学院牡丹江分院,黑龙江牡丹江157041

出　　处：《沈阳农业大学学报》2015年第4期385-390,共6页Journal of Shenyang Agricultural University

基　　金：长江学者和创新团队发展计划资助项目(IRT13079);北方粳稻育种与生产技术创新团队计划项目(201404235)

摘　　要：为了深入剖析水稻粒形性状的遗传调控机理,以典型籼稻品种七山占（Qishanzhan）和典型粳稻品种秋光（Akihikari）为亲本构建的重组自交系群体为材料,于2011-2013年分别对各株系的粒长、粒宽和粒厚3个粒形性状进行表型测定,并基于完备区间作图法（ICIM）进行粒形性状基因定位研究。试验结果表明：共检测到27个控制粒形性状的QTLs,包括3个粒长QTLs,11个粒宽QTLs和13个粒厚QTLs,它们分布于第1,2,3,4,5,11和12号染色体上,可分别解释14.45%-38.48%,28.98%-52.36%和38.77%-44.23%的表型变异;进一步分析发现,在第3,5和12号染色体上检测到的粒形性状QTL位点较多,且呈簇分布;此外,检测到q GL12a,q GL12b,q GW1,q GW5a,q GT11a和q GT12b等6个较新的QTLs位点,其中控制粒宽的q GW5a连续3年表达稳定,是一个重演性极好的QTL位点。以上结果将为水稻粒形性状的QTL克隆和遗传改良奠定基础。To analyze the genetic regulation mechanism of rice grain type traits, recombinant inbred lines（RILs） derived from a cross between Akihikari（typical japonica variety）/Qishanzhan（typical indica variety） were used to map QTL controlling seed shape（grain length, grain width and grain thickness） from 2011 to 2013 based on Inclusive Composite Interval Mapping（ICIM） method. A total of 27 QTLs were detected containing 3 QTLs for grain length, 11 QTLs for grain width and 13 QTLs for grain thickness, which were identified on chromosomes 1, 2, 3, 4, 5, 11 and 12 and accounted for 14.45%-38.48%, 28.98%-52.36% and 38.77%-44.23% phenotypic variation, respectively. Further analysis showed that three QTLs clusters were detected on chromosome 3, 5 and 12. In addition, six relatively new QTLs were found, such as q GL12 a, q GL12 b, q GW1, q GW5 a, q GT11 a and q GT12 b, and q GW5 a is one of good reproducibility QTL which controlling grain width,in three years. These conclusions would be the basis to the future research of QTL clone and molecular genetic improvement for rice grain shape.

关 键 词：水稻 重组自交系群体 粒形性状 数量性状基因位点 

分 类 号：S511[农业科学—作物学]