田间不同症状的高粱黑穗病病原菌种群鉴定  

Identification of the Pathogen Population of Sorghum Smut with Different Symptoms in Field

作　　者：姜钰[1,2] 徐婧[1,2] 徐秀德[1,2] 胡兰[1] 

机构地区：[1]辽宁省农业科学院植物保护研究所,沈阳11016 [2]沈阳农业大学植物保护学院,沈阳110161

出　　处：《沈阳农业大学学报》2015年第4期404-409,共6页Journal of Shenyang Agricultural University

基　　金：现代农业产业技术体系建设专项项目(CARS-06-02-01)

摘　　要：为了弄清田间多样的高粱黑穗病症状与病原种类间的关系,将采自我国北方高粱生产田中不同症状的高粱黑穗病菌,通过病菌培养性状与r DNA ITS序列分析,研究病菌种群多样性。结果表明:虽然高粱黑穗病症状差异很大,但病菌冬孢子萌发特点、菌落形态特征等无明显差异,且与高粱丝黑穗病的致病菌丝孢堆黑粉菌[Sporisorium reilianum(Kühn)Langdon&Fullerton]的特点相符合;供试菌株r DNA-ITS序列(序列号:KC986979)与Gen Bank中的丝孢堆黑粉菌(S.reilianum)的序列相似度达99%;田间高粱黑穗病的不同症状均由丝黑穗病的致病菌——丝孢堆黑粉菌侵染所致,并非属于其他黑粉菌种类。高粱黑穗病症状差异的原因尚有待于进一步研究。In order to clarify the relationship between the pathogen species and sorghum smut symptoms in the field, the pathogens of sorghum smut with different symptom from northeast sorghum production fields were identified based on the pathogens culture characteristics and r DNA-ITS sequence analysis. The results showed that the colony morphology and teliospore germination of pathogens were consistent with the characteristics of Sporisorium reilianum. The r DNA-ITS sequence（Accession No.KC986979） of tested strains showed a 99% identity to the sequence of S. reilianum that from Gen Bank. The pathogens of sorghum smut with different symptoms were belonging to the species of S. reilianum of sorghum head smut pathogen, and not belong to others. The cause of the difference symptoms of sorghum head smut are proposed for further research.

关 键 词：高粱 黑穗病菌 病害症状 ITS序列 

分 类 号：S435.14[农业科学—农业昆虫与害虫防治]