肝螺杆菌诱导不同品系小鼠慢性肝损伤与Th1/Th2细胞功能变化的分析  

Analysis of chronic liver injury induced by Helicobacter hepaticus in mice of different strains and functional changes of Th1/Th2 cells

作　　者：李红艳[1] 王静[1] 盛传伦[1] 赵晓南[1] 

机构地区：[1]吉林大学中日联谊医院,吉林长春130033

出　　处：《中国实验诊断学》2015年第12期2004-2008,共5页Chinese Journal of Laboratory Diagnosis

摘　　要：目的观察不同品系小鼠感染H.hepaticu后的肝脏病理损伤与Th1/Th2细胞功能的变化的关系。方法SPF级雄性BABL/c Cr、SCID/Cr、C57BL/6Cr小鼠接种H.hepaticus标准菌株ATCC51450菌液0.2 ml(1×108 CUF/ml),连续3次,每次间隔48h,对照组灌饲等量的PBS。于末次接种H.hepaticus后第16周处死小鼠,留取血清和肝脏,一份肝组织行病理组织学检查,另一份肝组织及血清应用ELISA方法检测细胞因子IFN-γ、TNF-α、IL-4、IL-5含量。结果 H.hepaticus感染后16周时BALB/c Cr小鼠血清、肝脏组织中IFN-γ、TNF-α含量明显高于对照组(P<0.001);BALB/c Cr小鼠血清、肝脏组织中IFN-γ、TNF-α含量明显高于SCID/Cr、C57BL/6Cr小鼠(P<0.001);而SCID/Cr小鼠、C57BL/6Cr小鼠血清、肝脏组织中IFN-γ、TNF-α含量与对照组相比无显著性差异(P>0.05);H.hepaticus感染后的各品系小鼠血清、肝脏组织中IL-4及IL-5含量与对照组相比,无显著性差异(P>0.05);H.hepaticus感染后BALB/c Cr小鼠血清及肝脏组织中IFN-γ、TNF-α含量随着肝脏病理评分的增高而增加(P<0.05;P<0.01)。结论 H.hepaticus感染BALB/c Cr小鼠后诱导慢性肝损伤的程度与Th1细胞因子IFN-γ、TNF-α的水平呈正相关;具有免疫活性小鼠H.hepaticus感染后宿主免疫反应是以Th1细胞为主的免疫反应。Objective To observe the relationship between liver pathological injury induced by H.hepaticus in mice of different strains and Th1/Th2 cell function.Methods SPF-class male BABL/c Cr、SCID/Cr,C57BL/6Cr mice inoculated H.hepaticus standard strain ATCC51450 0.2ml bacterial suspension（1×108 CUF/ml）,for 3times,each time interval of 48 hours,the control Group gavage the same amount of PBS.At last after Helicobacter hepaticus inoculation16 weeks mice were killed,specimens from serum and liver,one liver tissue for histopathology;application of ELISA method to detect cytokines IFN-γ,TNF-α,IL-4,IL-5contents of another liver tissue and serum.Results After H.hepaticus infection 16 weeks,IFN-γ,TNF-αcontents of serum and liver tissue in BALB/c Cr mice were significantly higher than the control group（P〈0.001）;which IFN-γ,TNF-αcontents of serum and liver tissue in BALB/c Cr mice were significantly higher than SCID/Cr mice and C57BL/6Cr mice（P〈0.001）;Compared with the control group,the IFN-γ,TNF-αcontents of serum and liver tissue in SCID/Cr mice and C57BL/6Cr mice after H.hepaticus infection were no significant difference（P〉0.05）;Compared with the control group,the IL-4,IL-5contents of serum and liver tissue in all strains of mice after H.hepaticus infection were no significant difference（P〉0.05）;IFN-γ,TNF-αcontents of serum and liver tissue in H.hepaticus infected BALB/c Cr mice were increased with liver pathology scores increased（P〈0.05;P〈0.01）.Conclusion The degree of chronic liver injury induced by H.hepaticus infection in BALB/c Cr mice were positively correlated with IFN-γ,TNF-αlevels of Th1cytokines;Host immune response after immunocompetent mice infect H.hepaticus is a Th1-based immune response.

关 键 词：肝螺杆菌 不同品系小鼠 慢性肝损伤 Th1/Th2细胞功能 

分 类 号：R285.5[医药卫生—中药学]