离子排斥色谱法对发酵液中的L-赤藓酮糖及赤藓糖醇的测定  

Determination of Erythritol and its Metabolites L-erythrulose from Fermentation Broth by Ion-exclusion Liquid Chromatography

作　　者：潘龙[1] 祁静[1] 靳魁奇[1] 郑小娟[1] 张磊[1] 刘宇鹏[1] 

机构地区：[1]河南大学生命科学学院生物工程研究所,河南开封475004

出　　处：《湖北农业科学》2015年第22期5719-5721,共3页Hubei Agricultural Sciences

摘　　要：采用离子排斥色谱法对赤藓糖醇及发酵产物L-赤藓酮糖进行了分析。以高交联度磺化苯乙烯-二乙烯基苯共聚物多孔微球作为固定相的Aminex HPX-87C离子色谱柱为分析柱,利用示差折光检测器分析了发酵液中赤藓糖醇和L-赤藓酮糖。通过色谱条件优化,选定了60℃柱温和30%（V/V）乙腈作为流动相。发酵液样品中赤藓糖醇和L-赤藓酮糖的平均回收率在97.2%~101.8%,精密度偏差在0.9%~2.4%范围内。A method using ion exclusion chromatography was described for the simultaneous determination of erythritol and its metabolites L-erythrulose as products of fermentation. Erythritol and L-erythrulose were analyzed by a sulfonated polystyrene divinylbenzene column （Aminex HPX-87C） with a refractive index detector. It was found that the optimalconditions for separation were 60 ℃ column temperature and 30% （V/V） acetonitrile as mobile phase,which applied to the quantification. The results showed that the recoveries were in the range of 97.2%~101.8% and the precision CV ranged from 0.9%to 2.4%.

关 键 词：L-赤藓酮糖 赤藓糖醇 示差折光检测器 离子排斥色谱法 

分 类 号：O657.75[理学—分析化学]