猪MBF1基因的克隆与序列分析  

Clonging and Sequence Analysis of Porcine MBF1 Gene

作　　者：武华玉[1] 程碧军[2] 乔木[1] 黄京书 刘贵生[1] 彭先文[1] 李良华[1] 吴俊静[1] 梅书棋[1] 

机构地区：[1]湖北省农业科学院畜牧兽医研究所,武汉430064 [2]湖北省农业科学院农业经济技术研究所,武汉430064 [3]湖北省畜牧兽医局,武汉430064

出　　处：《湖北农业科学》2015年第22期5748-5751,共4页Hubei Agricultural Sciences

基　　金：湖北省农业科技创新中心项目(2011-620-001-003);国家生猪产业技术体系项目(CARS-36);国家科技支撑计划项目(2011BAD28B01-13);动物胚胎工程及分子育种湖北省重点实验室开放课题(2012ZD143);湖北省重大人才工程高端人才引领培养计划项目

摘　　要：克隆了MBF1基因875 bp的c DNA序列和1 919 bp的基因全序列(登录号:EF 625885)。序列分析表明,猪MBF1基因序列与人、小鼠的相似性分别为89%和88%,氨基酸序列与人和小鼠的相似性分别为99%和97%,猪MBF1基因包含5个外显子和4个内含子,内含子的剪接方式都符合"GT-AG"法则;组织表达谱分析表明MBF1基因在心、肝、脾、肾、子宫中表达量较高,在胃和小肠中低表达。In this study, 875 bp mRNA sequence and 1 919 bp genomic sequence （EF 625885）of porcine MBF1 gene （multiprotein bridging factor 1） were cloned. A multiple alignment of MBF1 sequences showed that porcine MBF1 sequence had 89% identity with that of human, 88% identity with that of mouse, Amino acid sequence analysis showed that MBF1 had 99% identity with that of human, 97% identity with that of mouse. Sequence analysis revealed that porcine MBF1 gene consists of 5 exons and 4 introns and the locations of splice donor/aceeptor sites in all introns conform to ＂GT/AG＂ rule. RT- PCR analysis showed that MBF1 gene was highly expressed in heart, liver, spleen, kidney, uterus, and lowly expressed in stomach and small intestine.

关 键 词：猪 MBF1基因 克隆 序列分析 

分 类 号：Q78[生物学—分子生物学]