调心安神针法调节室性心律失常模型兔心肌细胞Cx43表达的免疫组化研究  

Immunohistochemical Study of Heart-regulating and Mind-calming Acupuncture for Adjusting Cx43 Expression in Myocardial Cells in a Rabbit Model of Ventricular Arrhythmia

作　　者：王锐[1] 郭丽[1] 李永春[2] 崔华峰[1] 管琳[1] 

机构地区：[1]山东中医药大学附属医院,济南250011 [2]山东中医药大学,济南250011

出　　处：《上海针灸杂志》2015年第12期1241-1243,共3页Shanghai Journal of Acupuncture and Moxibustion

基　　金：国家中医药管理局针灸重点学科重点项目;山东省中医药科技发展计划项目(2013ZDZK-014)

摘　　要：目的研究调心安神针法对于室性心律失常模型兔心肌细胞缝隙连接蛋白43(connexin43,Cx43)的影响。方法采用兔耳缘静脉注射氯化钡的方法制作室性心律失常模型。40只新西兰兔随机分为对照组、模型组、针刺组、药物组,针刺组施以调心安神针刺法(取灵台、神道及双侧内关、百会),药物组给予盐酸普罗帕酮片(心律平)灌胃。实验完毕后取兔左心室,通过免疫组织化学染色技术及计算机图像处理系统对Cx43的表达进行对比分析。结果模型组Cx43的表达较对照组显著降低(P<0.01),针刺组及药物组Cx43的表达均较模型组增加(P<0.05),二者之间无明显差异(P>0.05)。结论调节心肌细胞Cx43的表达可能是该针法治疗心律失常的靶点之一。Objective To investigate the effect of heart-regulating and mind-calming acupuncture on the expression of gap junction protein connexin43（Cx43） in myocardial cells in a rabbit model of ventricular arrhythmia. Method A rabbit model of ventricular arrhythmia was made by injecting barium chloride into the marginal ear vein. Forty New Zealand rabbits were randomized into control, model, acupuncture and medication groups. The acupuncture group received heart-regulating and mind-calming acupuncture [at Lingtai（GV10）, Shendao（GV11）, bilateral Neiguan（PC6） and Baihui（GV20）] and the medication group, oral gavage of propafenone. Rabbit left ventricle was taken after the completion of the experiment. Cx43 expressions were compared and analyzed using an immunohistochemical staining technique and a computer image processing system. Result Cx43 expression decreased significantly in the model group compared with the control group（P〈0.01） and increased in the acupuncture and medication groups compared with the model group（P〈0.05）. There was no significant difference in Cx43 expression between the acupuncture and medication groups（P〈0.05）. Conclusion Adjusting Cx43 expression in myocardial cells may be one of the target points for this acupuncture treatment of arrhythmia

关 键 词：针刺 调心安神针法 心律失常 室性 缝隙连接蛋白43 免疫组织化学法 

分 类 号：R2-03[医药卫生—中医学]