虎刺提取物对CCl_4致肝损伤的保护作用  

Protective Effect from Different Extracts of Damnacanthus indicus against CCl_4-induced Liver Injury in Mice

作　　者：王丹[1] 马瑞丽[1] 张蓉[1] 苏兆亮[2] 汤建[1] 欧阳臻[1] 

机构地区：[1]江苏大学药学院,江苏镇江212013 [2]江苏大学基础医学与医学技术学院,江苏镇江212013

出　　处：《中国野生植物资源》2015年第6期20-23,共4页Chinese Wild Plant Resources

基　　金：江苏省镇江市社会发展项目(SH2014062);博士后基金(2012M521023)

摘　　要：目的:研究虎刺提取物对CCl4致小鼠急性肝损伤的保护作用及与所含蒽醌类化合物之间的量效关系。方法:小鼠分为模型组,正常组,阳性药组(联苯双酯),石油醚部位组、乙酸乙酯部位组、水部位组和75%乙醇提取物组。正常组小鼠每日灌胃(ig)生理盐水10 m L·kg^(-1),阳性对照组联苯双酯200 mg·kg^(-1),药物组按300 mg·kg^(-1)(高剂量组)、150 mg·kg^(-1)(低剂量组)剂量给药。连续给药6天后小鼠按剂量10 m L·kg^(-1)腹腔注射(ip)0.1%CCl_4花生油溶液,心脏取血,测血清谷丙转氨酶(ALT),谷草转氨酶(AST)活性,并取肝脏做组织切片观察其病理学形态变化。采用高效液相(HPLC)法初步分析,采用醋酸-甲醇显色法测定各样品中总蒽醌的含量。结果:与模型组相比,虎刺水部位(300 mg·kg^(-1))能明显降低小鼠血清中ALT、AST的含量(p<0.01),水部位(150 mg·kg^(-1))和75%乙醇提取物(300 mg·kg^(-1))也能降低ALT、AST的含量(p<0.05)。病理切片发现水部位和总提物能明显减轻肝组织病理学症状。结论:虎刺提取物具有明显的肝保护作用,其中水部位活性最强,保护活性可能与蒽醌化合物有关。Aim： To study the protective effect from different extracts of Damnacan indicus against CCl_4-induced acute liver injury in mice,as well as the relationship with the contents of total anthraquinones.Methods： Mice were divided into 11 groups. Mice in model and normal groups were administrated（ ig）with saline at 10 m L·kg-（-1）daily. Mice in positive group were administrated（ ig） bifendate,200 mg·kg-（-1）. Tested groups were administrated（ ig） at 300 mg·kg-（-1）（ high dose group） and 150 mg·kg-（-1）（ low dose group）,respectively. After consecutive 6 days,the mice were administrated（ ip） with CCl_4 solution in peanut oil at 10 m L·kg-（-1）. The aspartate aminotransferase（ ALT）,alanine aminotransferase（ AST）levels in cardiac blood serum were tested. The liver biopsy morphology was evaluated. Each sample was analyzed for determining the content of total anthraquinones and chemical profile. Results： Water-soluble fraction（ 300 mg·kg-（-1）） could significantly deduced ALT,AST levels（ p 0. 01）. Water- soluble fraction（ 150 mg·kg-（-1）） and 75% ethanol extract（ 300 mg·kg-（-1）） could lower ALT,AST levels to some degree（ p 0. 05）. Water- soluble fraction and 75% ethanol extract could significantly reduce areas of necrosis of liver tissue. Conclusion： The water- soluble fraction had remarkable protective effects on liver injury,which could be associated with the contents of total anthraquinones.

关 键 词：虎刺 CCl4致肝损伤 总蒽醌 HPLC 

分 类 号：R285[医药卫生—中药学]