水产源气单胞菌对苯扎溴铵的药物敏感性分析  

Susceptibility of Aeromonas spp. from aquaculture against benzalkonium bromide

作　　者：田小龙[1,2] 张瑞泉[1] 姜兰[1] 邓玉婷[1] 谭爱萍[1] 罗理[1] 王伟利[1] 

机构地区：[1]中国水产科学研究院珠江水产研究所/农业部渔用药物创制重点实验室/广东省水产动物免疫技术重点实验室,广州510380 [2]上海海洋大学水产与生命学院,上海201306

出　　处：《南方农业学报》2015年第10期1903-1908,共6页Journal of Southern Agriculture

基　　金：公益性行业(农业)科研专项项目(201203085);广东省鱼病防治专项项目(2012)

摘　　要：【目的】了解水产源气单胞菌对苯扎溴铵的药物敏感性，为苯扎溴铵在水产养殖中的科学、合理使用提供参考依据。【方法】从广东省珠江三角洲地区某猪一鱼立体养殖鱼塘的猪粪、池塘水体、底泥及鱼体组织等样品中分离纯化气单孢菌，然后采用96孔微量板法对分离获得的气单孢菌进行最低抑菌浓度（MIC）和最低杀菌浓度（MBC）测定，分析其对苯扎溴铵的敏感性特征。【结果】共分离纯化获得121株气单胞菌，其中猪源64株、鱼源23株、池塘水源20株、池塘底泥源14株。苯扎溴铵对气单胞菌的MIC和MBC范围均在8-128μg／mL。在MIC50和MIC90方面，以猪源受试菌株的MIC50和MIC90最低，分别为8．9和30．0μg/mL；鱼源受试菌株的MIC50和MIC90最高，分别为22-3和39．2μg／mL。根据MBC／MIC≤4可判定药物具杀菌活性的原则，可知苯扎溴铵对99．17％受试菌株具有良好的杀菌效果。【结论】苯扎溴铵对水产源气单胞菌具有良好的杀菌效果，但在实际生产中需要提高其有效使用浓度。[Objective ]The susceptibility of Aeromonas spp. from aquaculture against benzalkonium bromide was stud- ied in order to provide references for scientific and rational use of benzalkonium bromide in aquaculture. [Method] Aeromonas strains were isolated from swine manure, pond water, sediment and fish of one pig-fish integrated pond locat- ed in Pearl River Delta area. And the minimal inhibitory concentration （MIC） and minimal bactericidal concentration （MBC） of these strains were determined by using 96-microwells plates, so as to analyze susceptibility of Aeromonas spp. against benzalkonium bromide. [Result]The results showed that a total of 121 Aeromonas strains were isolated and puri- fied, including 64 strains from swine, 23 strains from fish, 20 strains from pond water and 14 strains from sediment. The MIC and MBC of Aeromonas strains ranged from 8 to 128 μg/mL. MIC50 and MIC90 of strains from pig were 8.9 and 30.0 μg/mL, respectively, which were lower than those of strains from fish, water and sediment. Furthermore, MIC50 and MIC90 of strains from fish were the highest, which were 22.3 and 39.2 μg/mL, respectively. According to principle that if MBC/ MIC ratio was less than or equal to 4, drug would be judged to has bactericidal activity, the benzalkonium bromide had good bactericidal effect on 99.17% strains. [Conclusion]The benzalkonium bromide has good bactericidal effect on Aeromonas spp. from aquaculture, however its effective concentration should be increased in aquaculture.

关 键 词：气单胞菌 苯扎溴铵 药物敏感性 最低抑菌浓度(MIC) 最低杀菌浓度(MBC) 

分 类 号：S948[农业科学—水产养殖]