鲟病原性温和气单胞菌的分离鉴定及药敏试验  

Isolation,identification and drug sensitivity test of pathogenic Aeromonas sobria from sturgeon

作　　者：肖艳翼 王斌[2,3] 夏永涛[2,3] 胡利静 胡鲲[1] 杨先乐[1] 

机构地区：[1]上海海洋大学国家水生动物病原库,上海201306 [2]杭州千岛湖鲟龙科技股份有限公司,杭州310000 [3]中国水产科学研究院,北京100039

出　　处：《南方农业学报》2015年第10期1909-1914,共6页Journal of Southern Agriculture

基　　金：国家重大科技成果转化项目(ZD-2012-345-5);国家级星火计划项目(2014GA700003)

摘　　要：【目的】明确鲟鱼脓包病症的病因,为该病的防治提供参考依据。【方法】以常规方法从濒死鲟鱼病料中分离病原菌,纯化后进行回归感染试验,根据其形态、生理生化特征及分子生物学鉴定结果进行病原菌鉴定,并采用药敏纸片扩散法进行药敏试验。【结果】从患病鲟鱼的肌肉病灶组织、肠组织和腹水分离获得3株优势菌株(BX0814、BX0815和BX0816),经回归感染试验证实,BX0815菌株为致病菌;结合BX0815菌株的形态、生理生化特征、分子生物学鉴定结果,可确定BX0815菌株为温和气单胞菌(Aeromonas sobria)。药敏试验结果显示,BX0815菌株对痢特灵、磺胺异噁唑、氯霉素、多黏霉素、环丙氟哌酸、氟苯尼考、诺氟沙星、头孢噻肟、新霉素、卡那霉素、左氧氟沙星、链霉素、妥布霉素、庆大霉素、丁胺卡那霉素等15种药物高度敏感,而对万古霉素、阿莫西林、麦迪霉素、克林霉素、利福平、氨芐西林、青霉素等7种药物已产生了耐药性。【结论】引起鲟鱼患脓包病症的病原是温和气单胞菌,实际生产中可选用磺胺异噁唑、多黏霉素、氟苯尼考、诺氟沙星、头孢噻肟、新霉素、卡那霉素、左氧氟沙星、链霉素、妥布霉素、庆大霉素、丁胺卡那霉素等药物进行防治。[Object]The present experiment was conducted to investigate pathogenesis of sturgeon pustules, in order to provide references for prevention and treatment＇ of sturgeon pustules. [Method]The pathogenic bacteria were isolat- ed from dying sturgeon by conventional method. After being purified, the isolated bacteria was used for conducting recur- sire infection test, so as to identify pathogenic bacteria based on their morphology, physical and chemical characters, and molecular biology identification results, and the drug susceptibility was detected by disk diffusion method. [Result]Three dominant bacteria strains（BX0814, BX0815 and BX0816） were isolated from focal lesions, intestines and ascites. And strain BX0815 was found to be pathogenic based on results of reeursive infection test, besides, according to its morpholo- gy, physical and chemical characters, and molecular biology identification results, it was inferred that stain BX0815 was identified as Aeromonas sobria. The drug sensitivity test results showed that strain BX0815 was sensitive to furazolidone, sulfafurazole, chloramphenicol, polymyxin, ciprofloxacin, florfen＇icol, norfloxacin, claforan, neomycin, kanamycin, lev- ofloxacin, streptomycin, tobramycin, gentamycin and amikacin, while BX0815 was resistant to vancomycin, amoxicillin, medemycin, clindamycin, rifampicin, ampicillin and penicillin. [Conclusion]The pathogenic bacteria causing pustule symptom of sturgeon was A. sobria. And sulfafurazole, polymyxin, florfenicol, norfloxacin, claforan, neomycin, kanamycin, levofloxacin, streptomycin, tobramycin, gentamycin and amikacin can be used for preventing and treating pustules in sturgeon aquicuhure.

关 键 词：鲟 温和气单胞菌 分离 鉴定 药敏试验 

分 类 号：S965.215[农业科学—水产养殖]