紫外线对杉木体胚再生植株的影响  

Effect of Ultraviolet Radiation on Cunninghamia lanceolata Somatic Embryogenesis Plantlets

作　　者：吴华[1] 闫姗[1] 陈金慧[1] 郑仁华[2] 施季森[1] 成铁龙[1] 

机构地区：[1]南京林业大学林学院林木遗传与生物技术省部共建教育部重点实验室,江苏南京210037 [2]南京林业大学南方现代林业协同创新中心,江苏南京210037

出　　处：《林业科学研究》2015年第6期839-843,共5页Forest Research

基　　金：江苏省高校自然基金项目(No.13KJA220001);教育部新世纪优秀人才支持计划;江苏高校优势学科建设工程资助项目(PAPD);南京林业大学南方现代林业协同创新中心计划

摘　　要：为了解紫外线辐射时间对杉木生长的影响,本研究以杉木优良杂交组合的体胚再生植株为材料,用紫外灯模拟自然界UV-B辐射对其进行处理,研究0、1、3、5、7、9天紫外胁迫下杉木的光合作用效率及生理状态的变化。结果表明:随着紫外线辐射时间的延长,杉木体胚再生植株叶片的叶绿素含量明显降低、叶绿素荧光参数(Fv/Fm、YⅡ、q P、q N)数值均降低,MDA含量先降低后增加,POD活性提高、可溶性蛋白含量明显增加;紫外线辐射3天叶片杉木体胚再生植株叶片尖端开始变黄,5—7天黄化现象加剧,9天叶片完全失水,干枯死亡,说明长时间紫外辐射会阻碍杉木体胚再生植株的生长发育。To study the effect of ultraviolet radiation on Chinese fir （Cunninghamia lanceolata （Lamb.） Hook）, the somatic embryogenic plants （emblings） regenerated from Chinese fir were used as testing materials. The emblings were treated by different exposure days under ultraviolet light. The results showed that with the prolonging of exposure time under ultraviolet light, the chlorophyll concentration and chlorophyll fluorescence parameters decreased, while the MDA content increased with the activity of POD and soluble protein. It was revealed that the UV radiation could cause a large amount of MDA in leaf which may damage the cell membranes and further decrease the efficiency of photosynthesis of Chinese fir. Stresses may triggered the emblings responding pathway to protect dama- ges by UV radiation through activating POD competence and soluble protein, but failed to save the plants under sever damages in the investigation, POD may excited the activity soluble protein to protect emblings avoiding from damage caused by ultraviolet radiation, but could not being effectively alleviate.

关 键 词：杉木 紫外线 生理指标 叶绿素荧光参数 

分 类 号：S791.27[农业科学—林木遗传育种]