安徽省赤练蛇野生群体遗传多样性分析  

Analysis on Population Genetic Diversity of the Natural Chinese of Dinodon rufozonatum

作　　者：鲍方印[1] 肖明松[1] 王松华[1] 何庆元[1] 

机构地区：[1]安徽科技学院生命科学学院,安徽凤阳233100

出　　处：《安徽科技学院学报》2015年第5期16-19,共4页Journal of Anhui Science and Technology University

基　　金：安徽省高校自然科学研究重点项目(KJ2010A080)

摘　　要：利用mt DNA控制区(D-Loop)序列作为分子标记,对安徽省野生赤链蛇6个野生群体14个个体的遗传结构进行了分析。结果表明,赤链蛇D-Loop序列长690 bp,6个种群共检测到变异位点20个,占全部序列的3.42%,14个个体共检测到6种单倍型。采用邻接法(NJ法)对所有单倍型进行系统发育树构建,结果表明:安徽省野生赤链蛇可以分为3支,大别山区和皖南山区各1支,淮北、江淮丘陵及沿江为1支。In this study, the mitochondrial DNA control region （D- Loop） was used to analyze genetic diversity and structure of 6 natural populations of Dinodon rufozonatum collected from Huangzangyu, Tushan, Huangpush- an, Tiantangzhai, Jinshan and Hongcun separately. The results showed the length of this region （D -loop） con- tained 690 bp nucleotides and the A, C, T and G contents were 32.3%, 24.8%, 27.9% and 15.0% respec- tively. 20 nucleotide sites and 6 haplotypes were found in 6 wild populations of Dinodon rufozonatum. Molecular phylogenetic tree constructed by NJ method showed individuals from the different populations did not cluster to- gether. These results suggested wild Dinodon rufozonatum from Anhui could be divided into three branches, which were the Dabie Mountains and mountainous area of Southern Anhui, Huaibei, hilly areas between Yangtze River and Huaihe River and along the Yangtze River resoectively.

关 键 词：赤链蛇 MTDNA D-LOOP 遗传差异 

分 类 号：Q-9[生物学]