威廉环毛蚓耕作时间对菜田土壤微生物群落多样性及碳代谢特征的影响  

Effects of Pheretima Guillelmi Cultivation Time on Microbial Community Diversity and Characteristics of Carbon Metabolism in Vegetable Soil

作　　者：郑宪清[1,2,3,4] 范晓芬[4] 张翰林[1,2,3,4] 李双喜[1,2,3,4] 王金庆[1,2,3,4] 张娟琴[1,2,3,4] 王良军 陶晓斌 吕卫光[1,2,3,4] 

机构地区：[1]上海市农业科学院生态环境保护研究所,上海201403 [2]农业部上海农业环境与耕地保育科学观测实验站,上海201403 [3]上海东保农业科技有限公司,上海201403 [4]上海市设施园艺技术重点实验室,上海201403 [5]上海瀛西果蔬专业合作社,上海202158

出　　处：《农业资源与环境学报》2015年第6期596-602,共7页Journal of Agricultural Resources and Environment

基　　金：上海市农委成长计划项目(沪农青字(2015)第1-23号);上海市科委成果转化项目(133919N1400);上海市科技兴农重点攻关项目(沪农科攻字(2011)第6-2号)

摘　　要：通过在上海崇明岛菜田设置的定位试验,采用Biolog生态板法研究生物耕作时间对不同深度土层中土壤微生物群落多样性及其碳代谢特征的影响。3年的结果表明：生物耕作显著提高了微生物群落活性,且随着耕作年限的延长增加值越高,生物耕作3年可使平均AWCD值升高3～7倍。生物耕作处理Simpson指数和Shannon指数均显著高于免耕对照,在0～5 cm土层累积增幅分别为49和6.28,5～20 cm土层累积增幅分别为31和2.55。蚯蚓生物耕作显著增加土壤微生物对6类碳源的代谢能力,耕作3年碳水化合物代谢活性大幅增加。本研究说明蚯蚓生物耕作是增加土壤微生物代谢活性的有效途径。In order to study the effect of different biological tillage time （Pheretima guillelmi） on soil microbial community metabolic functions in different soil depths, we set a location test in vegetable field at Chongming Island in Shanghai to analyze the changes of soil microbial com- munity and carbon utilization abilities （Average well-color development, A WCD ） by using biolog eco-plate method. The three-year results showed that： Bio-tillage significantly improved microbial community activity, and with the increase of tillage years, biological tillage could make the average A WCD 3 to 7 times higher. The Simpson index and Shannon index of the biological tillage treatments were significantly higher than that of the control. The cumulative increase of 0-5 cm soil layer was 49 and 6.28 respectively, and the cumulative increase of 5- 20 cm soil layer was 31 and 2.55 respectively. Earthworm bio-tillage significantly increased the soil microbial metabolic ability of 6 kinds of carbon sources, and increased the carbohydrate metabolism activity. In this study, earthworm bio-tillage is an effective way to increase the microbial activity of microbial soil.

关 键 词：蚯蚓 生物耕作 土壤微生物 biolog生态板 碳代谢特征 

分 类 号：S181[农业科学—农业基础科学]