小剂量DMBA对大鼠胰腺的致瘤作用  

The effect of small dose DMBA on rat pancreatic tumorigenesis

作　　者：任宇[1,2] 侯晓朴[1,3] 李丹[1] 李颖[1] 朱斌[1] 

机构地区：[1]首都医科大学附属北京世纪坛医院普通外科,北京大学第九临床医学院,北京100038 [2]首都医科大学附属北京妇产医院乳腺外科,北京100006 [3]首都医科大学附属北京地坛医院介入科,北京100015

出　　处：《首都医科大学学报》2015年第6期936-941,共6页Journal of Capital Medical University

基　　金：北京市科学技术委员会首都市民健康项目培育项目(Z131100004013037)~~

摘　　要：目的探讨小剂量7,12-二甲基苯并蒽(7,12-dimethylbenzanthracene,DMBA)对大鼠胰腺的致瘤作用。方法将70只雄性SD大鼠采用数字表法随机分成2组:实验组60只,手术切开大鼠胰腺被膜按2 mg/100 g置入DMBA;对照组10只,仅行胰腺被膜的切开,不置入任何药物。2组术后每月称量大鼠体质量,并分别在手术后2、4、6个月取胰腺组织行病理学检查。结果1实验组大鼠6个月内体质量增长变化与对照组比较,差异无统计学意义(P=0.680)。2实验组大鼠术后4个月胰腺囊肿发生率为33.3%(6/18),6个月胰腺囊肿发生率38.9%(7/18),6个月胰腺部位平滑肌肉瘤发生率为5.6%(1/18),胰腺部位平滑肌肉瘤总发生率1.8%(1/55);无胰腺导管细胞癌发生。结论小剂量DMBA(2 mg/100 g)置入SD大鼠6个月后可引起大鼠急性胰腺炎、慢性胰腺炎及胰腺囊肿,无胰腺导管细胞癌发生;胰腺肉瘤发生率很低,原因有待进一步研究。Objective To explore the possibility of pancreatic carcinogenesis induced by small dose 7,12-dimethylbenzanthracene（ DMBA） implantation in Sprague-Dawley（ SD） rats. Methods Totally 70 male SD rats were randomly divided into two groups： DMBA（ 2 mg/100 g） was implanted into the parenchyma of rats pancreas in experimental group（ n = 60）; No drugs were implanted into the parenchyma of pancreas in control group. Rats were killed after surgery operation（ 2,4 and 6 months）. Finally,the pancreases of rats were fixed in formalin for HE staining and immunohistochemical analysis. Results ① No significant differences were observed with the weight of rats between experimental and control groups（ P = 0. 680）; ② The incidence of pancreatic cyst was 33. 3% in the experimental group 4 months after surgery. The incidence of pancreatic cyst was 38. 9% and that of leiomyosarcoma was 5. 6% in the experimental group 6 months after surgery. Conclusion Small dose of DMBA（ 2 mg /100 g） implanted into SD rats can cause acute pancreatitis,chronic pancreatitis,pancreatic cyst,and pancreatic leiomyosarcoma. The incidence of pancreatic leiomyosarcoma is very low. But no pancreatic ductal cell carcinoma was found. Further reaserch is needed.

关 键 词：胰腺肿瘤 肉瘤 7 12-二甲基苯并蒽(DMBA) 动物模型 

分 类 号：R322.4[医药卫生—人体解剖和组织胚胎学]