反式-4-羟基-L-脯氨酸手性固定相的制备及性能评价  

Preparation and eevaluation of trans-4-hydroxy-L-proline chiral stationary phase

作　　者：陈玲[1] 李扬[1] 农蕊瑜 艾萍[1] 赵慧玲[1] 袁黎明[1] 

机构地区：[1]云南师范大学化学化工学院,昆明650500

出　　处：《分析试验室》2015年第12期1365-1369,共5页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金项目(21365025)资助

摘　　要：合成并表征了反式-4-羟基-L-脯氨酸硅胶键合手性配体交换色谱固定相,研究了其在高效液相色谱中的分离能力,取得了较为满意的效果—拆分了7种手性化合物、氯酚及二硝基苯两种位置异构体,并探讨了多方面因素(如流动相p H、柱温、流速等)对分离效果的影响,得到了更佳的色谱分离条件。A chiral stationary phase bonding trans-4-hydroxy-L-proline to silica gel was successfully synthesized for ligand exchange chromatography. The chiral column was used for the separations of some racemates and isomers with different mobile phase on high performance liquid chromatography. The experimental results showed that 7 kinds of enantiomer and 2 kinds of isomer were separated. The influences of the mobile phase pH, temperature of column and flow rate of eluent on the resolution of enantiomers and isomers were also investigated for the optimization of chromatographic conditions.

关 键 词：反式-4-羟基-L-脯氨酸 手性配体交换色谱固定相 手性分离 高效 液相色谱 

分 类 号：TQ016[化学工程] O657.7[理学—分析化学]