正交设计优选复方熊胆茵陈颗粒水提醇沉工艺研究  

Technology research of water extracting-alcohol precipitating of compound Xiongdan Yinchen particles by orthogonal design

作　　者：阙金花 黄鸣清[1] 郑海音[2] 徐伟[1] 李煌[1] 洪振丰[2] 许舒瑜[1] 刘杰[1] 林诗瑶 

机构地区：[1]福建中医药大学药学院,福建福州350122 [2]福建中医药大学中西医结合学院,福建福州350122

出　　处：《山西中医学院学报》2015年第5期20-26,共7页Journal of Shanxi College of Traditional Chinese Medicine

基　　金：福建省科技重大专项(2010YZ0001-1)

摘　　要：目的:优选复方熊胆茵陈颗粒的水提醇沉工艺条件。方法:以绿原酸、柴胡皂苷a的含量和浸膏得率为考察指标,正交试验法优选水提醇沉最佳工艺条件。结果:最佳水提醇沉条件为加8倍量水提取2次,每次1.5 h;醇沉药液相对密度1.2(50℃),醇沉乙醇浓度为70%,醇沉时间24 h。结论:该优选工艺方法简便、稳定可行,可作为复方熊胆茵陈颗粒的提取纯化工艺。Objective：To optimize technology conditions of water extracting-alcohol precipitating of compound Xiongdan Yinchen particles. Methods：Orthogonal test was adopted and the contents of chlorogenic acid,saikosaponin a and the yield of extractum were as indices. Results：The optimum technology conditions were as follows：8 times quantity of water extracts 2 times,1 times per 1.5 h;the relative density of alcohol precipitation solution was 1.2 （50℃）,the concentration of alcohol was 70%,and the alcohol precipitation time was 24 h. Conclusion：The optimization method is simple and stable,could be used as the technology of extraction and purification of compound Xiongdan Yinchen particles.

关 键 词：复方熊胆茵陈颗粒 水提醇沉 正交试验 绿原酸 柴胡皂苷A 

分 类 号：R284.2[医药卫生—中药学]