CMTM5基因rs723840单核苷酸多态性与阿司匹林治疗下血小板高反应性的相关性研究  

Association between CMTM5 gene rs723840 single nucleotide polymorphism and high on asprin platelet reactivity

作　　者：刘滕飞[1] 张婧薇 陈夏欢[1] 冯雪茹[1] 柏中胜 刘梅林[1] 

机构地区：[1]北京大学第一医院老年内科,北京100034

出　　处：《北京大学学报（医学版）》2015年第6期905-909,共5页Journal of Peking University:Health Sciences

基　　金：国家国际科技合作专项项目(2013DFA30860)资助~~

摘　　要：目的：探讨趋化素样因子超家族成员5（CKLF-like MARVEL transmembrane member 5,CMTM5）基因rs723840单核苷酸多态性与阿司匹林治疗下血小板高反应性的相关关系。方法：采用临床单组观察试验研究设计方法,共入选210例北京大学第一医院老年内科住院患者,采用光学比浊法测定0.5 g/L花生四烯酸（arachidonic acid,AA）诱导的血小板聚集率（platelet aggregation ratio,PR）,阿司匹林治疗下血小板高反应性（high on aspirin platelet reactivity,HAPR）定义为PR≥3/4 PR界值。将入选患者按照PR分为HAPR组和非血小板高反应性组（NoHAPR）。采用焦磷酸测序法测定CMTM5基因rs723840单核苷酸多态性在HAPR组和No-HAPR组的基因型及等位基因分布频率,采用卡方检验统计分析基因型与等位基因在两组间的差异。结果：CMTM5基因rs723840多态在两组间基因型分布频率皆符合Hardy-Weinberg平衡定律,3种基因型（CC型、CT型和TT型）分布频率在HAPR组和No-HAPR组分别为48.4%、51.6%、0.0%和73.7%、22.9%、0.034%,3种基因型在两组间分布频率差异存在统计学意义（P=0.004）,C、T等位基因分布频率在两组间差异亦有统计学意义（P=0.031,OR=2.002,95%CI：1.056~3.793）。Logistic回归校正了年龄、性别、体重指数、吸烟、高血压、糖尿病及高脂血症等冠心病易患因素后,CMTM5基因rs723840单核苷酸多态性仍与冠心病患者HAPR的发生存在显著相关性。结论：CMTM5基因单核苷酸多态位点rs723840与冠心病患者血小板高反应性发生存在显著相关性。Objective： To elucidate the correlation between the single nucleotide polymorphism of CKLF-like MARVEL transmembrane member 5（ CMTM5） gene rs723840 and the occurrence of high on aspirin platelet reactivity（ HAPR）. Methods： The present study is a case-control study. A total of 210 hospitalized patients in Peking University First Hospital were enrolled. Aspirin response was assessed by0. 5 g / L arachidonic acid（ AA）-induced platelet aggregation ratio（ PR）,and ≥3 /4 quartile of PR of the population was defined as HAPR. Accordingly all the enrolled 210 coronary artery diseases（ CAD）patients were divided into HAPR group and No-HAPR group. The genotypes were determined by polymerase chain reaction（ PCR） and sequencing analysis for rs723840 of CMTM5 gene. Results： The genotype frequencies in rs723840 C 〉T of CMTM5 gene conformed well to the Hardy-Weinberg equilibrium in both HAPR group and No-HAPR group. Between the two groups,the genotypes frequencies in HAPR and No-HAPR groups were 48. 4%,51. 6%,0. 0% and 73. 7%,22. 9%,0. 034%,respectively（ P =0. 004）. The C,T allele frequencies were significantly different in the two groups（ P = 0. 031,OR =0. 501,95% CI： 0. 264-0. 947）. Conclusion： Our study finds a significant correlation between CMTM5 gene rs723840 polymorphism and high on aspirin platelet reactivity.

关 键 词：血小板聚集 CMTM5 多态性 单核苷酸 阿司匹林 

分 类 号：R541.4[医药卫生—心血管疾病]