血府逐瘀汤含药血清对Toll样受体4信号转导通路及下游炎症因子的影响  

Study of Xuefu Zhuyu Decoction anti-atherosclerotic mechanism

作　　者：姜玉姬[1] 姜华[1] 

机构地区：[1]延边大学附属医院,延吉133000

出　　处：《中国免疫学杂志》2015年第12期1633-1636,共4页Chinese Journal of Immunology

基　　金：国家自然科学基金项目(81160476)资助

摘　　要：目的:研究血府逐瘀汤含药血清对血管内皮细胞Toll样受体4信号转导通路及下游LOX-1、TNF-α、VCAM-1及ICAM-1等炎症因子表达的影响,探讨血府逐瘀汤抗动脉粥样硬化的机制。方法:采用药物血清学方法制备血府逐瘀汤含药血清以备细胞实验使用。体外培养血管内皮细胞,随机分为对照组、模型组、阿托伐他汀组、血府逐瘀汤组,用LPS刺激2 h后,分别加入阿托伐他汀和血府逐瘀汤含药血清干预24 h。用荧光定量PCR方法测定TLR4、MYD88、TRAF-6、NF-κB、LOX-1、TNF-α、VCAM-1及ICAM-1的基因表达;用Western blot方法测定TLR4、NF-κB、LOX-1、TNF-α、VCAM-1及ICAM-1蛋白表达。结果:用LPS刺激血管内皮细胞后,引起TLR4、My D88、TRAF-6、NF-κB、LOX-1、TNF-α、VCAM-1及ICAM-1的表达升高,与对照组比较,差异有统计学意义(P<0.01);用药物血清干预以后血府逐瘀汤组显著抑制各项指标的表达,与模型组比较,差异有统计学意义(P<0.05)。结论:血府逐瘀汤可抑制TLR4/NF-κB信号转导通路及下游LOX-1、TNF-α、VCAM-1及ICAM-1等炎症因子的表达,这可能是其防治动脉粥样硬化作用的机制之一。Objective： To investigate the influence of Xuefu Zhuyu Decoction（ XZD） contained Drug-Serum on the expression of TLR4 / NF-κB signals and LOX-1,TNF-α,ICAM-1,VCAM-1 in HUVECs,and to study its possible anti-atherosclerotic mechanism. Methods： HUVECs was cultured in vitro and divided equally into the normal group,the model group,the ATV group and the XZD group in random. HUVECs were stimulated with LPS for 2 h,then treated separately with the drug-serum and atorvastatin for 24 h,finally measured the expression of TLR4,My D88,TRAF-6,NF-κB,LOX-1,TNF-α,ICAM-1 and VCAM-1 mRNA with real-time PCR,the expression of TLR4,NF-κB,LOX-1,TNF-α,ICAM-1 and VCAM-1 protein were analyzed by Western blot method. Results：Protein and mRNA expressions of TLR4,My D88,TRAF-6,NF-κB,LOX-1,TNF-α,ICAM-1 and VCAM-1 increased significantly after LPS stimulation（ vs normal control group,P〈 0. 01）,Xuefu Zhuyu Decoction decreased the high expression of TLR4,My D88,TRAF-6,NF-κB,LOX-1,TNF-α,ICAM-1 and VCAM-1（ vs model group,P〈 0. 05）. Conclusion： Xuefu Zhuyu Decoction can block the high expression of TLR4 and its downstream signal transduction pathway and the high expression of LOX-1,TNF-α,ICAM-1 and VCAM-1. Maybe it＇s the mechanism of Xuefu Zhuyu Decoction exert the function of anti-artherosclerosis.

关 键 词：血府逐瘀汤 TOLL样受体4 LOX-1 动脉粥样硬化 

分 类 号：R2-031[医药卫生—中西医结合]