2,9-双甲基苯并噻唑乙烯基邻菲咯啉与DNA的相互作用及应用研究  

Interaction of a Phenanthroline Derivative with DNA and Its Application

作　　者：常昂 刘祥军[1] 吴尚荣[1] 柳影[1] 王林林[1] 张楠[1] 李松青[2] 上官棣华[1] 

机构地区：[1]北京分子科学国家实验室中国科学院化学研究所活体分析化学院重点实验室,北京100190 [2]湘潭大学化学学院,湘潭411100

出　　处：《化学通报》2015年第12期1101-1101,共1页Chemistry

摘　　要：设计合成了基于2,9-双苯并噻唑乙烯基取代的邻菲咯啉荧光分子PMBT。通过吸收光谱、荧光光谱、圆二色谱探讨了PMBT与不同DNA的相互作用,发现PMBT与DNA存在两种不同的相互作用模式。由于PMBT分子中带有2个正电荷,当PMBT与DNA的浓度比值较高(大于4)时,PMBT以DNA为模板按一定方向在DNA上聚集;当PMBT与DNA的浓度比小于2时,PMBT通过嵌插或末端堆积的方式分别与单/双链DNA和G-四链体DNA结合。PMBT与DNA结合导致其荧光猝灭,利用该特性将PMBT与DNA结合构建荧光增强型检测平台,可用于DNA酶活性以及DNA降解的动力学研究。A phenanthroline derivative,2,9-bis{ 2-（ 3-methylbenzo[d]thiazol-2-yl） vinyl}-1,10-phenanthroline（ PMBT）,was designed and synthesized. The interaction of PMBT with different DNA forms was investigated by UV-Vis absorption spectrum,fluorescence spectrum and circular dichroism spectrum. PMBT possesses a large conjugated planar structure,and binds to DNA with different modes dependent on the molecular ratio of PMBT and DNA. At high molecular ratio（ 4）,PMBT binds to DNA through DNA-templated aggregation because of the two positive charges on each of PMBT. At low molecular ratio（ 2）,PMBT binds to DNA through intercalation between the DNA bases or end-stacking on G-quadruplexes. PMBT has good solubility in aqueous solution,and exhibits strong fluorescence. The fluorescence is quenched when bound to DNA. Thus PMBT was used as a fluorescent probe for investigation of DNA degradation in serum and the activity of nuclease.

关 键 词：邻菲咯啉衍生物 DNA 相互作用 荧光探针 

分 类 号：O641.3[理学—物理化学]