HPLC法对钩藤超临界萃取物中钩藤碱的分析  

Study on the HPLC Method for Analysis of Rhynchophylline Extracted from Uncaria tomentosa by Supercritical Fluid

作　　者：曾启华[1,2] 李森柱[1] 王蒙[1] 林锦良[1,2] 

机构地区：[1]遵义师范学院化学化工学院,贵州遵义563002 [2]贵州省黔北特色资源应用研究实验室,贵州遵义563002

出　　处：《湖北农业科学》2015年第21期5398-5400,共3页Hubei Agricultural Sciences

基　　金：遵义市科技局社会发展攻关项目(遵市科合社字[2009]17号);贵州省教育厅"125"重大科技专项(黔教学重大专项字[2012]015号)

摘　　要：通过超临界二氧化碳萃取技术(SFE-CO_2)和高效液相色谱法(HPLC),以甲醇-水(70∶30,V/V)为流动相,对钩藤碱进行分析。研究表明,方法回归方程为y=7.000 0×10^(-8)x-0.000 6(R^2=0.994),即钩藤碱浓度在0.015~0.022 mol/m L内呈良好的线性关系。该分析方法快速、简单、灵敏,具有良好的准确度和精密度,可用于钩藤碱常规分析检验。The analysis of rhynchophylline by the means of supercritical carbon dioxide extraction technology and high perfor-mance liquid chromatography （HPLC） analytic method with methanol and water （70：30,V/V ） as mobile phase. The results showed that regression equation for y=7.000 0×10-8x-0.000 6 （R2=0.994）, and the linear relationship was good in the region of 0.015～0.015 mol/mL. The analysis method was rapid, simple, sensitive and has good accuracy and precision, can be used for routine analysis of rhynchophylline.

关 键 词：HPLC法 超临界萃取 钩藤[Uncaria rhynchophylla(Miq.)Jack.] 钩藤碱 

分 类 号：O629.3[理学—有机化学]