氟在中华圆田螺体内富集及其对肝胰脏免疫相关酶的影响  

Tissue distribution of fluoride and its effects on immunerelated enzymes activities of hepatopancreas in Cipangopaludina cahayensis

作　　者：李潇[1] 靳文博[1] 李若静 祝欢欢 赵晓晓[1] 曹谨玲[1] 

机构地区：[1]山西农业大学动物科技学院/生态畜牧与环境兽医学省级重点实验室,山西太谷030801

出　　处：《广东农业科学》2015年第21期124-128,共5页Guangdong Agricultural Sciences

基　　金：国家自然科学基金(31440087;31172376;31372497);中国博士后科学基金(2012M520601;2013T60267);山西农业大学科研启动基金(XB2007 003);山西农业大学博士后基金(92462);山西农业大学科技创新基金(2009005);山西农业大学第十二批大学生创新创业训练项目(12-13);山西省高等学校大学生创新创业训练项目(2014096)

摘　　要：以中华圆田螺为试验材料,研究水环境中不同浓度(0、40、80、160 mg/L)氟暴露条件下,氟在其体内的富集及对肝胰脏免疫相关酶,如酸性磷酸酶(ACP)、碱性磷酸酶(AKP)和过氧化氢酶(CAT)活性的影响。结果显示,在不同浓度氟化钠连续暴露30、60 d后,与对照组比较,不同浓度氟暴露组中华圆田螺不同组织中的氟含量均显著高于对照组,且随着氟暴露剂量的升高,呈现先升后降趋势,表明氟在中华圆田螺不同组织中的浓度已经达到平衡;在整个染毒过程中,染毒组ACP和AKP活性与对照组相比呈先降后升的趋势。氟暴露30 d,高浓度组ACP和AKP活性极显著高于对照组,氟暴露60 d,所有染毒组ACP和AKP活性均显著低于对照组;染毒组CAT活性与对照组相比呈先升后降再升趋势,且80 mg/L组和160 mg/L组CAT活性极显著低于对照组。Tissue distribution of fluoride and its effects on the activities of immune-related enzymes（ACP,AKP and CAT）were examined in hepatopancreas of Cipangopaludina cahayensis exposed to different concentrations of fluoride for 60 days. The results showed that the fluoride accumulation in different tissues after exposed to fluoride significantly increased compared with the control group and the trend which increased and then decreased with the level and duration of exposure was found. Stead state was achieved under the experimental conditions. The activities of ACP and AKP first decreased and then increased during the fluoride exposure. After 30 days of exposure,the activities of ACP and AKP in hepatopancreas exposed to the highest concentration of fluoride highly significantly elevated compared with the control group. After exposure for 60 days,the activities of ACP and AKP in all fluoride-exposed C. cahayensiswere remarkably lower than those in the control mudsnail. The CAT activity increased first and then decreased and highly significantly decreased in mudsnail exposed to 80 mg/L and 160 mg/L of fluoride relative to the control during the whole exposure.

关 键 词：氟 中华圆田螺 生物富集 免疫相关酶 

分 类 号：X503.225[环境科学与工程—环境工程]