家蝇蝇蛆耐高温抗氧化多肽的筛选及性质分析  

Screening and characteristics of antioxidant peptides with thermostability from housefly maggot

作　　者：王土连 张晟[1] 黄绮薇[1] 柯德森[1] 

机构地区：[1]广州大学生命科学学院,广东广州510006

出　　处：《广东农业科学》2015年第21期145-149,共5页Guangdong Agricultural Sciences

基　　金：广东省自然科学基金(2014A030313531);广东省专业综合改革试点项目(生物工程;粤教高函[2013]113号);广州市科技计划项目(201510010108);广州大学国家级大学生创业训练项目(2015)

摘　　要：将家蝇(Musca domestica)蝇蛆进行不同高温的水浴处理,测量蝇蛆抗氧化性的变化。结果显示,50℃处理抗氧化活力变化不显著,80、100℃处理短时间内出现显著下降,但残留的抗氧化活力在随后的高温中保持稳定,提示家蝇蝇蛆存在耐高温抗氧化物质。将蝇蛆提取液在100℃处理不同时间以筛选耐高温抗氧化物质,结果表明,经100℃处理5 min筛选到的抗氧化物质在50、80、100℃保温5 h,甚至在120℃超高温条件下保温30 min,其抗氧化活力均保持稳定。SDS-PAGE电泳结合三氯乙酸沉淀反应结果证明,该物质为分子量约为30 ku的多肽。The maggots of Musca domestica were bathed in different temperatures of water,and the change of antioxidant activity was measured. The results showed that antioxidant activity had no significant change bathing at 50℃,while that at 80℃ and 100℃ had significant decline in a short time,then remained stable,which suggested that housefly bots have high temperature resistant antioxidant material. The maggot extracts were bathed at 100℃ for different time,and the antioxidant activity was measured to screen antioxidant material with thermostability. The results showed that the residual antioxidant substances after treatment at 100℃ for 5 min,could remain stable at 50℃,80℃,100℃ for 5 h,even under the condition of 120℃ for 30 min. Comprehensive analysis combined with SDS-PAGE electrophoresis and trichloroacetic acid precipitation showed that the antioxidant material was a 30 ku peptide.

关 键 词：蝇蛆 抗氧化多肽 耐高温 NBT法 

分 类 号：Q599[生物学—生物化学]