菌剂强化潜流湿地总氮总磷去除及功能菌特性  

Nitrogen and phosphorus removal and characteristics of functional microbes in subsurface flow wetland with microbe augmentation

作　　者：陈晶[1] 张敏特 陈萍[1] 邓文[1] 周新程 张文艺[1] 

机构地区：[1]常州大学环境与安全工程学院,常州213164 [2]江苏科技大学土木工程和建筑学院,镇江212005

出　　处：《环境化学》2015年第12期2268-2274,共7页Environmental Chemistry

基　　金："十二五"国家水体污染控制与治理科技重大专项(2012zx07301-001);国家自然科学基金(41571471);常州市科技支撑计划资助

摘　　要：从活性污泥中筛选的1株具有高效反硝化能力的聚磷菌B8应用于水平潜流人工湿地中进行强化去除总氮和总磷试验,同时分析并对比了菌剂强化潜流湿地系统和未投菌潜流湿地系统的功能菌数量变化规律.采用常绿植物构建2套相同的水平潜流湿地尾水处理模拟生态系统.结果表明,连续投加14 d B8菌液于水平潜流湿地后,在停止投菌后运行89 d内,投菌湿地系统平均脱氮率为70.1%,未投菌湿地系统平均脱氮率为50.2%;在停止投菌后运行19 d内,投菌湿地系统平均除磷率为63.1%,未投菌湿地系统平均除磷率为45.9%.经过4个月的跟踪运行,基于高通量454测序对湿地微生物群落结构及相关生物学信息对比分析,表明投加外菌源B8会引起潜流湿地内部微生物物种数量减少、微生物均匀度下降和湿地基质微生物群落多样性下降.通过湿地进水总氮浓度对投菌湿地系统和未投菌湿地系统脱氮率影响线性拟合分析表明,投菌湿地在不同氮负荷条件下脱氮效果显著优于未投菌湿地,证实投加B8菌可以有效强化水平潜流湿地的脱氮能力.A polyphosphate-accumulating organism（ B8） with high denitrifying ability was isolated from activated sludge to strengthen removal of total nitrogen and total phosphorus in horizontal subsurface flow constructed wetland. At the same time,the variation in the quantity of functional microbes in subsurface flow wetland with technology of microbes augmentation was analysed. The simulation ecosystem of horizontal subsurface flow constructed wetland in tailwater treatment was constructed by the evergreen plant,and a control group was designed without adding B8 stain agent.Culturing B8 stain agent and starting up the horizontal subsurface flow constructed wetland at the same time by taking the tailwater as influent and by the condition of self-inoculation for 14 consecutive days. The results showed that after 89 d,the mean removal rate of TN was 70. 1% by completion of inoculation,while it was 50.2% in the control group. Simultaneously,it also showed that after 19 d,the mean removal rate of total phosphorus was 63.1% by completion of inoculation,while it was 45. 9% in the control group. After 4 months of tracking operation,samples were collected in the wetlands,and analyzed using high-throughput 454 sequencing to comparativelyanalyze the microbial structures and related biological information. The analysis showed that the microbial species number,microbial uniformity and microbial community diversity all decreased under the treatment of B8 addition when compared to the control group. The linear fitting analysis of influence of influent total nitrogen concentration on denitrifying effectiveness show that total nitrogen removal ability was greatly improved after completion of inoculation. The study confirmed that it was possible to strengthen total nitrogen removal efficiency in horizontal subsurface flow constructed wetland by adding B8 stain agent.

关 键 词：潜流湿地 反硝化聚磷菌 投菌 功能菌 脱氮 

分 类 号：X703[环境科学与工程—环境工程] X172