ret和ednrb在先天性巨结肠症及巨结肠类源病肠壁表达的研究  

Expression of ret and ednrb in intestinal wall of Hirschsprung′s disease and Hirschsprung′s allied disease patients

作　　者：张怡先 张利兵 闫焕 钟麟[2] 

机构地区：[1]成都市妇女儿童中心医院外一科,四川成都610000 [2]四川大学华西附属第一医院小儿外科,四川成都610041

出　　处：《四川生理科学杂志》2015年第4期178-183,共6页Sichuan Journal of Physiological Sciences

摘　　要：目的:通过探讨ret、ednrb基因在先天性巨结肠症(Hirschsprung disease,HD)及巨结肠类缘病(Hirschsprung allied disease,HAD)患者肠壁的表达分布情况,旨在进一步了解HD与HAD发病机制的异同,为HD与HAD的诊断及治疗提供新的研究方向。同时通过常规HE染色与免疫组化标记时HD与HAD诊断的比较,深入了解免疫组化标记技术在HD和HAD诊断中的临床价值。方法:复习巨结肠术后病例HE切片,结合光镜下肠壁的形态特征,初步诊断分组。应用蛋白基因产物9.5(Protein gene product 9.5,PGP9.5)和神经元特异性烯醇化酶(Neuron specific enolase,NSE)免疫组化技术对经HE染色病理初步诊断为HD,HAD和不明确组的病变肠段再次诊断分组。HD肠段分别取狭窄段和扩张段与10例正常结肠行RET抗体、EDNRB抗体免疫组化染色,比较RET和EDNRB的染色结果和分布特点。结果:HE染色8例诊断不明确者,经免疫组化标记后诊断明确;RET在HD狭窄段中表达较HAD和正常对照组减少(P<0.05);EDNRB在HD狭窄段中表达较HAD和正常对照组减少(P<0.05);HD和HAD扩张段与正常组的RET与EDNRB表达差异无统计学意义(P>0.05)。结论:免疫组化染色有助于HD和HAD的诊断;RET和EDNRB在HD狭窄段中表达有异常,提示RET和EDNRB与HD的发生可能有关。Objective：Through comparing the expression of ret andednrbgene in the intestinal wall of patients with Hirschsprung’s disease and Hirschsprung’s allied disease,to investigate the differences between HD and HAD’s pathogenesy and indicate the potential direction of HD and HAD’s diagnosis and treatment.Meanwhile,to collect further information of the value of the immunohistochemistry in diagnosis of HD and HAD.Methods：Reviewed the HE slides of the patients who suffered the resection of megacolon under the inverted light microscope,diagnosed primarily and divided into groups by character of intestine wall.The PGP9.5and NSE immunohistochemistry assays were used to diagnose the cases secondly.The expression of ret andednrbingene in each group was detected by the immunohistochemistry assay.Results：Eight cases which were indeterminately diagnosed by HE,definitively diagnosed by immunohistochemistry.Ret was low expressed in HD stegnosis,compared with HAD and control groups（P〈0.05）.Ednrbwas low expressed in HD stegnosis,compared with HAD and Control groups（P〈0.05）.No expression difference of ret and ednrbin gene between HD and HAD extensive intestine and normal intestine.Conclusion：The immunohistochemistry assay could assist the diagnosis of HD and HAD.The abnormal expression ret and ednrbin gene in HD stegnosis,indicated those two genes may have the potential relationship with occurrence of HD.

关 键 词：先天性巨结肠症 巨结肠同源病 蛋白基因产物9.5 神经元特异性烯醇化酶 受体型酪氨酸激酶基因 内皮素B受体基因 免疫组化 

分 类 号：R726.5[医药卫生—儿科]