精氨酸抗利尿激素对急性肺损伤大鼠肺水肿液的清除作用  

Effect of arginine vasopressin on alveolar fluid clearance in rats with acute lung injury

作　　者：邓旺[1] 王导新[1] 

机构地区：[1]重庆医科大学附属第二医院呼吸内科,重庆400010

出　　处：《南方医科大学学报》2015年第11期1602-1605,共4页Journal of Southern Medical University

基　　金：国家自然科学基金(81270141)~~

摘　　要：目的探讨精氨酸抗利尿激素(AVP)对急性肺损伤肺水肿液清除作用。方法 48只健康成年的雄性SD大鼠随机分为对照组、模型组(ALI组)、AVP组,观察各组肺组织病理形态学、肺水含量、肺泡上皮通透性及肺泡液体清除率(AFC)变化,测定肺泡上皮钠通道(ENa C)和钠/钾ATP酶(Na+,K+-ATPase)表达情况。结果经AVP治疗后,模型组肺泡上皮通透性(0.27±0.15 vs0.59±0.19)及肺水含量(5.01±1.59 vs 8.67±1.79)减轻,AFC增加(23.56±4.51 vs 8.28±3.57),α-ENa C(1.296±0.322 vs 0.349±0.141)和α1-Na+,K+-ATPase表达增加(1.421±0.389 vs 0.338±0.186),均有显著差异(P<0.05)。结论 AVP能促进AFC,其作用途径可能是上调α-ENa C和α1-Na+,K+-ATPase通道蛋白实现的。Objective To investigate the effect of arginine vasopressin（AVP） on alveolar fluid clearance（AFC） in acute lung injury（ALI）. Methods Forty-eight healthy adult Sprague-Dawley rats were randomly divided into control group, ALI model group and AVP treatment group. The pathological changes in the lungs, lung water content, alveolar permeability and AFC were observed, and the expressions of alveolar epithelial sodium channel（ENa C） and Na＋, K＋-ATPase were measured. Results Compared with those in the model group, the rats treated with AVP showed significantly decreased alveolar permeability（0.27±0.15 vs 0.59±0.19） and lung water content（5.01±1.59 vs 8.67±1.79）（P〈0.05） and increased AFC（23.56±4.51 vs 8.28±3.57） and of α-ENa C expressions（1.296 ＋ 0.322 vs 0.349 ± 0.141） and α1-Na＋, K＋-ATPase（1.421 ± 0.389 vs 0.338 ± 0.186）（P〈0.05）. Conclusion AVP can promote AFC in with ALI possibly by up-regulation of α-ENa C, α1-Na＋, and K＋-ATPase.

关 键 词：精氨酸抗利尿激素 上皮钠通道 钠/钾ATP酶 急性肺损伤 肺泡液体清除 

分 类 号：R563.8[医药卫生—呼吸系统]