两种不同厂家ELISA试剂检测抗-HIV结果的差异性分析  

Analysis of the differences between the results of two ELISA kits in the detection of anti-HIV

作　　者：朱浩稳[1] 徐飞[1] 徐国锋[1] 李勇[1] 邹国英[1] 任碧琼[1] 

机构地区：[1]湖南省第二人民医院/湖南中医药大学临床医学院,长沙410007

出　　处：《检验医学与临床》2015年第24期3629-3630,共2页Laboratory Medicine and Clinic

基　　金：国家卫计委医药卫生科技发展中心科技资助项目(28-6-7);湖南中医药大学省级优秀团队建设资助项目(2013);湖南省第二人民医院重点专科基金资助项目(2012)

摘　　要：目的探讨不同厂家酶联免疫吸附试验(ELISA)试剂检测人免疫缺陷病毒抗体(抗-HIV)结果的差异性。方法同时用两种不同厂家生产的第3代ELISA抗-HIV初筛试剂检测日常标本,初筛阳性结果的标本再用原来的试剂双孔复检排除假阳性,复检阳性的标本送确认实验室做免疫印迹试验(WB试验)确认。结果用A、B两种试剂共检测30批次2 166例标本,经复检A、B两种试剂仍为阳性的标本数分别为9例和19例,其中A、B两种试剂共同阳性反应的标本6例;WB试验确认2例阳性,为A、B两种试剂同时阳性。单独A试剂的阳性预测值为22.22%(2/9),单独B试剂的阳性预测值为10.53%(2/19),两种试剂双阳性标本的阳性预测值为33.33%(2/6)。结论不同厂家ELISA试剂检测抗-HIV结果的阳性预测值有较大差异,同时采用两种试剂检测临床标本能提高阳性预测值。Objective To explore the differences of the results of ELISA kits provided by different manufacturers in the anti-HIV detection.Methods Two kinds of third generation ELISA kits for anti-HIV screening provided by different manufacturers were used for routine sample detection.For the samples with positive results in screening,the original reagents were used to retest with double-hole,in order to eliminate false-positive.And the samples with positive results in retest were sent to confirmation laboratory to confirm the results by Western blot（WB）.Results 2 166 samples of 30 batches were respectively detected by using reagent A and B.After retest,there were 9and 19 cases of positive samples respectively detected by reagent A and B,and among them there were 6cases of samples with positive results detected both by reagent A and B.There were 2cases of positive samples confirmed by WB,which were positive detected both by reagent A and B.The positive predictive values of reagent A and B were22.22%（2/9）and 10.53%（2/19）respectively.The positive predictive value of both of reagent A and B was 33.33%（2/6）.Conclusion The positive predictive values of different anti-HIV ELISA kits from different manufacturers were significantly different.And the positive predictive value could be improved by using both of reagent A and B.

关 键 词：酶联免疫吸附试验 抗体 人免疫缺陷病毒 阳性预测值 

分 类 号：R446.6[医药卫生—诊断学]