肉鸡白念珠菌病常用实验诊断方法比较研究  

Comparison research on commonly experimental diagnostic methods of candidiasis albicans in broiler chicken

作　　者：刘建钗[1] 柳焕章[1] 刘彦威[1] 王慧真[1] 张永英[1] 

机构地区：[1]河北工程大学农学院动物医学系,邯郸056021

出　　处：《中国人兽共患病学报》2015年第12期1151-1156,共6页Chinese Journal of Zoonoses

基　　金：河北省自然科学基金资助项目(No.C2015402157)~~

摘　　要：目的探究肉鸡白色念珠菌病简便快速诊断方法。方法对50例来自不同鸡场的临床疑似样本分别采用KOH镜检法、改良KOH镜检法、分离纯化与形态观察法、TTC显色培养法、组织切片法和巢式PCR法进行试验。结果上述方法的样本检测阳性率分别为62.0%（31/50）、78.0%（39/50）、82.0%（41/50）、82.0%（41/50）、86%（43/50）和88.0%（44/50）,耗时分别为1~2h、1~2h、7~8d、6~7d、8~10d和7~8h。结论通过比较各种常用方法的优缺点,建议采用巢式PCR法与改良KOH镜检法相结合作为肉鸡白念珠菌病简便快速诊断的优选方案,其特异性强,敏感度高,耗时少,并兼顾病原侵染状态的直观性。In order to study handy and quick diagnosis of candidiasis albicans of broiler chicken,assays using 50 clinical suspected samples from several chicken farms were performed by methods of KOH microscopic examination,modified KOH microscopic examination,morphological observation,TTC chromogenic culture,histologic section technique,and nested PCR technique.The positive rates of detected samples by methods above were 62.0%（31/50）,78.0%（39/50）,82.0%（41/50）,82.0%（41/50）,86%（43/50）and 88.0%（44/50）,and the time drains were 1-2hours（h）,1-2h,7-8d,6-7d,8-10 dand 7-8h,respectively.By comparison of merits and demerits between different methods,aproposal of combining the nested PCR test with the modified microscopic examination is presented as a preferred embodiment for handy and quick diagnosis of candidiasis albicans in broiler chicken,which has advantages including high specificity and sensitivity,less time consumption and taking account of visualizability of the invasive pathogen.

关 键 词：肉鸡 白念珠菌 诊断方法 巢式PCR 

分 类 号：R378.99[医药卫生—病原生物学] S854.4[医药卫生—基础医学]