连续多次应用丙泊酚复合维生素C对小鼠麻醉效应的影响  

Anesthetic effects of repeated dosing with propofol and vitamin C in mice

作　　者：郭梦倬 李伟光[2] 刘旭[1] 冯泽国[1] 张成岗[2] 吴芳勇[1] 黄连军[1] 于颖群[3] 

机构地区：[1]解放军总医院麻醉手术中心,北京100853 [2]军事医学科学院放射与辐射医学研究所,北京100850 [3]解放军第307医院麻醉科,北京100071

出　　处：《南方医科大学学报》2015年第12期1701-1704,共4页Journal of Southern Medical University

基　　金：国家自然科学基金(81371232);国家科技重大专项(2014ZX09J14107-05B)~~

摘　　要：目的探讨连续6 d给予丙泊酚或丙泊酚配伍维生素C对小鼠的麻醉效果。方法取40只昆明鼠随机分为丙泊酚80 mg/kg组（P80组）、丙泊酚70 mg/kg＋维生素C50 mg/kg组（P70＋Vc50组）、丙泊酚55 mg/kg＋维生素C100 mg/kg组（P55＋Vc100组）、丙泊酚50 mg/kg＋维生素C200 mg/kg组（P50＋Vc200组）,共4组,各组小鼠10只,以腹腔注射给药的方式,在同一时间点连续6 d给药,观察并记录小鼠麻醉诱导时间以及维持时间,并进行比较。结果与同天的丙泊酚组（P80）相比,给药的第1、2、3天的P55＋Vc100组、P50＋Vc200组麻醉维持时间缩短（P〈0.05）。与同一组第1天麻醉维持时间相比,各组小鼠其余5 d麻醉维持时间均缩短（P〈0.01）;与同一组第2天麻醉维持时间相比,第3天P50＋Vc200组麻醉维持时间缩短（P〈0.01）,各组小鼠第4、5、6天麻醉维持时间均缩短（P〈0.01）。各组小鼠翻正反射消失的发生率由第1~6天逐渐下降,且各组趋势接近一致。结论维生素C与丙泊酚配伍,可在保证麻醉效果的前提下一定程度减少丙泊酚使用剂量,同时可减少丙泊酚耐受的发生,对于减少丙泊酚麻醉剂量相关的副作用或不良反应。Objective To explore the anesthetic effects of repeated administration of propofol combined with vitamin C in mice.Methods Forty mice were subjected to daily intraperitoneal injections of 80 mg/kg propofol（P80 group）, 70 mg/kg propofol and 50 mg/kg vitamin C（P70 ＋ Vc50 group）, 55 mg/kg propofol and 100 mg/kg vitamin C（P55 ＋ Vc100 group）, or 50 mg/kg propofol and 200 mg/kg vitamin C（P50 ＋ Vc200 group） for 6 consecutive days, and the anesthesia induction time and anesthesia duration were recorded. Results Compared with the P80 group, the mice in P55 ＋ Vc100 group and P50 ＋ Vc200 group showed significantly shorter anesthesia duration on the first 3 days（P〈0.05）. In all the groups, anesthesia duration was significantly shortened in the following days compared with that on day 1（P〈0.01）; anesthesia duration was shorter on day 3than on day 2 in P50 ＋ Vc200 group（P〈0.01）, and was shorter on days 4, 5, and 6 than on day 2 in all the groups（P〈0.01）. In all the groups, the rate of loss of righting reflex（LORR） decreased gradually with time in a similar pattern. Conclusions Vitamin C can reduce the dose of propofol without obviously affecting the anesthetic effect to reduce the incidence of drug tolerance and potential dose-related side effects of propofol.

关 键 词：丙泊酚 维生素C 麻醉 翻正反射消失 

分 类 号：R614[医药卫生—麻醉学]