真武汤对大部分肾切除所致尿毒症心肌病小鼠心脏的保护作用  

Protective effects of Zhenwutang on cardiac function in mice with uremic cardiomyopathy induced by subtotal nephrectomy

作　　者：陈琪[1] 李志樑[1] 刘北[1] 傅强[1] 严全能[1] 

机构地区：[1]南方医科大学珠江医院心血管内科,广东广州510280

出　　处：《南方医科大学学报》2015年第12期1725-1728,共4页Journal of Southern Medical University

基　　金：国家自然科学基金(81573732);广东省自然科学基金(2015A030313304);广东省科技计划项目(2014A020212544)~~

摘　　要：目的探讨真武汤对小鼠尿毒症心肌病心脏保护作用及其机制。方法大部分肾切除术制作尿毒症心肌病模型。C57BL/6小鼠随机分3组:假手术组、模型组和真武汤组。真武汤组小鼠术后予真武汤灌胃,假手术组和模型组小鼠术后予等量蒸馏水灌胃。心脏彩超、心脏组织HE染色及血生化分别观察尿毒症心肌病小鼠心脏功能、结构及肾功能变化,Western blotting检测心脏组织p-AMPK及p-mTOR的表达情况。结果模型组小鼠较假手术组体质量明显下降(P<0.05),心脏质量及心脏指数明显增加(P<0.05),心脏舒张期及收缩期左室后壁厚度增大(P<0.05),HE染色示心肌细胞肥大,血尿素氮、肌酐显著升高(P<0.05)。真武汤组小鼠与模型组相比心脏质量及心脏指数减小(P<0.05),心脏舒张期及收缩期左室后壁厚度减小(P<0.05),病理变化减轻。模型组小鼠与假手术组相比p-AMPK表达明显降低,p-mTOR表达明显升高(P<0.05);真武汤组小鼠与模型组相比pAMPK表达明显升高,p-mTOR表达明显降低(P<0.05)。结论真武汤抑制尿毒症心肌病小鼠心肌肥厚,此效应可能是通过AMPK-mTOR信号通路实现。Objective To investigate the protective effects of Zhenwutang（ZWT） on cardiac function in mice with uremic cardiomyopathy（UCM）. Methods Thirty C57BL/6 mice were randomized into 3 equal groups, including a sham- operated group, subtotal nephrectomy（UCM model） group and subtotal nephrectomy with ZWT treatment group. The mice in the former two groups were treated with distilled water. The changes in cardiac functions, myocardial structure and renal function of the mice were evaluated with echocardiography, HE staining and biochemical assay, respectively. Western blotting was used to detect the expression level of adenosine monophosphate- activated protein kinase（AMPK） and mammalian target of rapamycin（mTOR） in the heart tissues. Results Compared with the sham- operated group, the mice in the model group showed significantly lowered body weight and increased heart weight, heart index, left ventricular posterior wall thickness in diastole（LVPWd） and in systole（LVPWs）, blood urea nitrogen（BUN） and serum creatinine（Scr）（P〈0.05）; Pathological examination revealed myocardial hypertrophy in the model group with markedly decreased expression levels of p-AMPK and significantly increased p- mTOR expression（P〈0.05）. ZWT treatment significantly decreased the heart weight, heart index,LVPWd, and LVPWs and expression level of p- mTOR（P〈0.05）, increased the expression level of p- AMPK（P〈0.05）, and obviously ameliorated histological injury of the heart in mice with UCM. Conclusion ZWT can protect the cardiac function in mice with subtotal nephrectomy-induced UCM possibly via the AMPK-mTOR signal pathway.

关 键 词：真武汤 尿毒症心肌病 AMPK-m TOR 

分 类 号：R285.5[医药卫生—中药学]