基于叶绿体DNA分析7种丁香的遗传多样性  

Studys on Genetic Diversity of Seven Syringa on Chloroplast DNA Variations

作　　者：刘焕臻[1] 刘悦[2] 韩青[2] 冉文容 齐羽彤 

机构地区：[1]东北林业大学林木遗传育种国家重点实验室,黑龙江哈尔滨150040 [2]东北林业大学林学院,黑龙江哈尔滨150040

出　　处：《林业科技》2015年第6期1-4,共4页Forestry Science & Technology

基　　金：中央高校基本科研业务费专项资金项目(DL13BAX11)

摘　　要：对5种常见的丁香及2种新发现的金叶丁香叶绿体DNA的3个保守序列进行测序,共获得1 722 bp,发现多态性位点18个,atp B-rbc L区域的多态性位点最多,trnL-trnF区域次之。分析七种丁香的核苷酸多样性为0.004 72,每个位点的突变率为0.004 55。3个保守区域中,trnL-trnF区域的核苷酸多样性和突变率都高于其他2个区域,说明在这几种丁香中,trnL-trnF区域变异性最大,核酸多样性和突变率最高。In this study several common lilac（Syringa） and two of the newly discovered golden leaf lilac were used, three conserved regions of the chloroplast DNA were sequenced and obtained 172 2bp. There were 18 polymorphic sites in total sequences, with the largest number of polymorphic sites were found in atp B-rbc L spacer, and trnL-trnF spacer was followed by. Nucleotide diversity was 0.004 72, and mutation rate per site was 0.004 55. Among three conserved regions, the trnL-trnF spacer showed the largest nucleotide diversity and mutation rate. This indicated that trnL-trnF spacer had the most DNA variations in seven Syringa. Further analysis of phylogenetic relationships were also obtained, these provided strong molecular evidence for the genetic relationships of seven Syringa, and also provided the molecular basis for the two newly discovered golden leaf Syringa further research.

关 键 词：丁香 DNA变异性 叶绿体保守序列 

分 类 号：S685.26[农业科学—观赏园艺] S603[农业科学—园艺学]