鱼丸中DHA含量测量不确定度评定  

Measurement Uncertainty Evaluation of Docosahexenoic Acid in Fish Ball

作　　者：张凤枰[1,2,3] 曹静[3] 杜雪莉 刘耀敏 王锡昌[3] 

机构地区：[1]通威股份有限公司检测中心,成都610041 [2]通威股份有限公司水产富禽营养与健康养殖农业部重点实验室,成都610041 [3]上海海洋大学食品学院,上海201306

出　　处：《中国食品学报》2015年第11期234-239,共6页Journal of Chinese Institute Of Food Science and Technology

基　　金：四川省科技支撑计划项目(No.2011NZ0071)

摘　　要：对采用气相色谱内标法测定鱼丸中的二十二碳六烯酸(DHA)含量的测量不确定度进行评定。通过建立测定过程中各分量的数学模型,分析、识别不确定度来源。其不确定度主要来源于校正因子、样品称量、十一碳酸甘油三酯内标溶液、重复测定、回收率等因素。在对各不确定度分量进行量化的基础上,合成得到测定结果的标准不确定度。当鱼丸的DHA含量为170.2 mg/100 g时,其合成标准不确定度和扩展不确定度分别为2.7 mg/100 g和5.4 mg/100 g。重复测定引入的不确定度分量对总不确定度的贡献最大。十一碳酸甘油三酯内标溶液浓度及添加体积、回收率、校正因子带来的不确定度分量也不容忽视。试样称量对总不确定度的贡献最小。本研究结果为采用该方法测定鱼丸中DHA含量提供理论依据。In this paper, the measurement uncertainty for determination of docosahexenoic acid in fish ball with gas chromatography by using internal standard method was evaluated. The measurement model of each component in the process of measurement was established. The main sources of uncertainty were analyzed and identified. The impact of every uncertainty component for the measurement uncertainty was estimated. Based on the quantitative analysis of every source,the relative standard uncertainty of measurement result was obtained. The determination result of docosahexenoic acid content in fish ball was 170.2 mg/100 g, the combined standard measurement uncertainty and expanded measurement uncertainty were 2.7 mg/100 g and 5.4 mg/100 g respectively. The evaluation indicated that repetitive test made the most contribution to the combined relative standard measurement uncertainty. The influence of concentration of internal standard solution, the added volume of internal standard solution, the spike recovery and correction factor could not be neglected.The effect of sample weight was minimal. This evaluation provided a theoretical basis for the quality control of docosahexenoic acid content determination in fish ball.

关 键 词：测量不确定度评定 气相色谱 内标法 二十二碳六烯酸(DHA) 鱼丸 

分 类 号：O657.71[理学—分析化学] TS254.7[理学—化学]