黄连素抑制人喉癌Hep-2细胞增殖及机制研究  

Berberine on proliferation of human laryngeal Hep-2 carcinoma cells

作　　者：李蕾[1] 丁小青[1] 周建业[1] 周卫东[1] 汤夏冰[1] 

机构地区：[1]南京医科大学附属无锡人民医院,江苏无锡214023

出　　处：《长春中医药大学学报》2015年第6期1109-1111,1144,共4页Journal of Changchun University of Chinese Medicine

基　　金：南京医科大学科技发展基金(2013NJMU161;2013NJMU167)

摘　　要：目的探讨黄连素对人喉癌Hep-2细胞增殖的抑制作用及其可能机制。方法体外培养Hep-2细胞,使用不同浓度黄连素诱导不同时间,应用MTT法检测细胞增殖抑制率;流式细胞术测定细胞周期及凋亡;实时荧光定量PCR和蛋白质免疫印迹(Western blot)法分别检测细胞周期蛋白D(Cyclin D)、B细胞淋巴瘤/白血病-2(Bcl-2)、Bcl-2相关X蛋白(Bax)基因和蛋白表达水平。结果与0μmol/L黄连素对照组比较,2.5、5.0、10.0、20.0μmol/L黄连素作用Hep-2细胞24、48 h,可明显抑制细胞增殖,且呈浓度时间依赖性(P<0.05);2.5、5.0、10.0μmol/L黄连素作用Hep-2细胞48 h,细胞早期凋亡率明显升高,呈浓度依赖性(P<0.05);G0/G1期细胞增多(P<0.05),诱导细胞阻滞于G0/G1期;10μmol/L黄连素作用Hep-2细胞48 h,Bax基因和蛋白表达水平明显升高(P<0.05),Cyclin D、Bcl-2基因和蛋白表达水平明显下降(P<0.05)。结论黄连素能抑制喉癌细胞增殖,引起G0/G1期阻滞,并诱导其凋亡,其作用机制可能与Bax基因表达上调及Cyclin D、Bcl-2基因表达下调有关。Objective To investigate the effect of berberine on proliferation of human laryngeal Hep-2 carcinoma cells and its possible mechanism. Methods The Hep-2 cells were treated with different concentrations of berberine for different hours,the proliferation inhibitory rates were detected by MTT assay; the cell cycle and apoptosis were analyzed by flow cytometry; the levels of Cyclin D,Bcl-2 and Bax were measured by real-time PCR and Western blot. Results The proliferation inhibition rates of Hep-2 cells treated with 2. 5、5. 0、10. 0、20. 0 μmol/L berberine for 24 and 48 hours were increased compared with 0μmol/L group in concentration-and time-dependent manners（ P〈0. 05）. The early apoptotic rate was increased in concentration-dependent manner（ P〈0. 05）,and the proportion of cells in G0 /G1 phase was increased after treated with 2. 5,5. 0,10. 0 μmol/L berberine for 48 hours（ P〈0. 05）. The levels of Bax m RNA and protein expression significantly increased（ P〈0. 05）,while the levels of Cyclin D and Bcl-2 expression significantly decreased（ P〈0. 05）. Conclusion Berberine can inhibit proliferation,and induct cell cycle G0 /G1 arrest and apoptosis of Hep-2 cells probably via upregulation of Bax and downregulation of Cyclin D and Bcl-2 m RNA and protein expression.

关 键 词：黄连素 喉癌 细胞增殖 

分 类 号：R767[医药卫生—耳鼻咽喉科]