枸杞沼泽红假单胞菌转化液中辅酶Q_(10)的含量测定  

Determination of Coenzyme Q_(10) in Lycium barbarum Rhodop Seudanonas palustris Conversion Solution

作　　者：刘亚妮[1] 杨飞[1] 郑庆红[1] 张志平[1] 杨官娥[1] 

机构地区：[1]山西医科大学药学院,太原030001

出　　处：《数理医药学杂志》2016年第1期3-5,共3页Journal of Mathematical Medicine

基　　金：太原市科技项目人才专项明星专题(120247-08);国家自然科学基金项目(81173473);山西省自然科学基金项目(2010011048-2);2012年山西省高等学校大学生创新创业训练项目(201231

摘　　要：目的：建立枸杞沼泽红假单胞菌转化液中辅酶Q10 HPLC含量测定方法.方法：采用C18色谱柱（4.6mm×150mm,5μm）,流动相：甲醇：无水乙醇（35∶65）,流速：1.0mL/min;检测波长：275nm;柱温：25℃;进样量：20μL.结果：该方法专属性良好,线性范围为5.0-30μg/mL（r=0.9996,n=6）;最低检测限为2.4ng,定量限为12ng;平均回收率为97.7% （n=6）,RSD为2.0%.结论：该法快速、简便、灵敏、准确,适合于枸杞沼泽红假单胞菌转化液中辅酶Q10的含量测定.Objective:To determine a method to determine the content of Coenzyme Q10 in Lycium barbarum Rhodopseudomonas palustris bio-converting extract.Methods:C18(4.6mm ×150mm,i.d.5μm)was adopted;Mobile phase :methanol∶anhydrous ethanol(35∶65);flow rate:1.0 mL/min;detection wave length :275nm;column temperature:25 ℃;sample size:20μL.Results:The method was specific,and the linear curve was within 5~30μg/mL(r=0.9996,n=6);The minimum detection limit was 2.4ng,quantitation limit was 12ng;The average recovery rate was 97.7% and RSD was 2.0%(n=6).Conclusion:The method is rapid,simple,sensitive,accurate it is applicable to determine the content of Coenzyme Q10 in Lycium barbarum Rhodopseudomonas palustris bio-converting extract.

关 键 词：枸杞 沼泽红假单胞菌 HPLC 辅酶Q10 含量测定 

分 类 号：R917[医药卫生—药物分析学]