红菜薹小孢子培养技术研究  

Study on Microspore Culture Techniques in Purple Flowering Stalk(Brassica campestris)

作　　者：吴艺飞[1] 丁茁荑[1] 戴雄泽[1] 周晓波[1] 汪端华[1] 

机构地区：[1]湖南省蔬菜研究所/湖南省蔬菜工程技术研究中心,长沙410125

出　　处：《作物研究》2016年第1期73-77,共5页Crop Research

基　　金：现代农业产业技术体系专项(CARS-25)

摘　　要：以5个红菜薹品种为试材,从花蕾的选择、影响小孢子出胚的关键因素、胚状体成苗及生根条件等方面对红菜薹小孢子培养技术进行了系统研究.结果表明：小孢子培养取样最佳时期为单核靠边期,瓣萼比在0.626～0.834之间;基因型不同对小孢子培养影响很大,以H13-3出胚率最高,为2.78个/皿;胚状体诱导的最适培养基为Keller-13添加6-BA1.0mg/L＋KT1.0mg/L＋NAA0.5mg/L,出胚率为2.67个/皿,且70%以上为子叶形胚;转接胚状体最适宜的胚龄为15～25d,成苗率在65% ～83%;再生植株生根培养基添加NAA最适浓度为0.4mg/L,侧根多而粗壮,移栽成活率高.With five purple flowering stalk varieties as test materials,the microspore culture techniques in purple flowering stalk were systematically studied from some aspects,such as choice of buds,key factors affecting on microspore embryogenesis,seedling formation of embryoid and rooting conditions,and so on. The results showed that the optimum sampling period of microspore culture was the late uninucleate stage and 0. 626 ~ 0. 834 sepal than. Genotype had great influence on microspore culture,and embryogenesis rate of H13- 3 was highest,2. 78 / dish. The optimum culture medium embryoid for induction was Keller-13 with 6-BA 1. 0 mg / L + KT 1. 0 mg / L + NAA 0. 5 mg / L,and the embryogenesis ratewas 2. 67 /dish,and more than 70% was cotyledon embryo. The optimum embryonic age to transfer embryoid was 15 ~ 25 d,and the seedling rate could reach to 65% ~ 83%. The regeneration optimal concentration of NAA added into rooting medium was0. 4 mg / L,which could gain more thick lateral root and higher transplanting survival rate.

关 键 词：红菜薹 小孢子培养 胚状体 出胚率 

分 类 号：S634.6[农业科学—蔬菜学]