Development and characterization of synthetic amphiploids of Oryza sativa and Oryza latifolia  

作　　者：Chuandeng Yi Mingsen Wang Wei Jiang Derong Wang Xiaojun Cheng Ying Wang Yong Zhou Guohua Liang Minghong Gu 

机构地区：[1]Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops, Key Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou University

出　　处：《Science Bulletin》2015年第23期2059-2062,共4页科学通报（英文版）

基　　金：supported by the National Natural Science Foundation of China (31571624, 31071382);the National Basic Research Program of China (2010CB125904, 2013CBA01405);the key Natural Science Project in University of Jiangsu Province (15KJA210004);the Priority Academic Program Development of Jiangsu Higher Education Institutions

摘　　要：Oryza sativa and Oryza latifolia belong to the AA and CCDD genomes of Oryza, respectively. In this study, amphiploids were obtained from the tube seedlings of O. sativa × O. latifolia F1 hybrids by treatment with colchicine, an agent for chromosome doubling. Subse- quently, amphiploids were investigated using the methods of morphology, genomic in situ hybridization, and molec- ular markers. Amphiploids were characterized by a shorter plant height, larger diameter of stem, longer and wider leaves, darker leaf color, decreased spikelets per panicle and panicle length, and larger spikelets and anthers than the original F1 hybrid. Based on the mitotic metaphase chro- mosome number of the investigated root tips, the somatic chromosome number of the amphiploid is 2n = 72. Additionally, the amphiploid is an allohexaploid, and its genomic constitution is AACCDD by genomic in situ hybridization analysis. Finally, the amphiploids were identified to be true using 37 polymorphic markers at the DNA level.Oryza sativa and Oryza latifolia belong to the AA and CCDD genomes of Oryza, respectively. In this study, amphiploids were obtained from the tube seedlings of O. sativa 9 O. latifolia F1 hybrids by treatment with colchicine, an agent for chromosome doubling. Subsequently, amphiploids were investigated using the methods of morphology, genomic in situ hybridization, and molecular markers. Amphiploids were characterized by a shorter plant height, larger diameter of stem, longer and wider leaves, darker leaf color, decreased spikelets per panicle and panicle length, and larger spikelets and anthers than the original F1 hybrid. Based on the mitotic metaphase chromosome number of the investigated root tips, the somatic chromosome number of the amphiploid is 2n = 72.Additionally, the amphiploid is an allohexaploid, and its genomic constitution is AACCDD by genomic in situ hybridization analysis. Finally, the amphiploids were identified to be true using 37 polymorphic markers at the DNA level.

关 键 词：Oryza sativa · Oryza latifolia· Colchicine · Amphiploid · Genomic in situhybridization 

分 类 号：S511[农业科学—作物学]