改良阴茎海绵体穿刺法在大鼠阴茎海绵体内压测定中的应用研究  

Application of a modified intracavernous cannulation in monitoring the intracavernous pressure of rats

作　　者：赵善坤[1] 康然[1] 刘路浩[1] 黎富添 李二毛 罗连民 赵志刚[1] 

机构地区：[1]广州医科大学附属第一医院泌尿外科男科中心广东省泌尿外科重点实验室,510230

出　　处：《中华泌尿外科杂志》2015年第12期941-945,共5页Chinese Journal of Urology

摘　　要：目的 探讨改良阴茎海绵体穿刺法在大鼠阴茎海绵体内压（intracavernous pressure,ICp）测定中的应用效果.方法 2014年10-12月将20只3个月龄雄性SD大鼠按随机数字法随机分为常规阴茎海绵体穿刺组（采用阴茎脚部位穿刺）及改良阴茎海绵体穿刺组（穿刺部位为阴茎海绵体中段）,每组10只.测定ICP与平均动脉压（mean arterial pressure,MAP）的比值（ICP/MAP）,评价大鼠勃起功能.电刺激参数均为电压5V,频率15 Hz,波宽5 ms,刺激时间60s.每只大鼠测定3次取平均值.结果 常规阴茎海绵体穿刺组与改良组所测得的基础ICP分别为（14.9±2.7）、（15.5±3.1） mmHg（1 mmHg =0.133 kPa）;刺激后ICP分别为（87.6±7.5）、（85.0±6.2） mmHg;ICP/MAP分别为（71.3±6.6）％、（72.5±6.3）％,组间比较差异均无统计学意义（P＞0.05）.改良法能在直视下快速准确穿刺阴茎海绵体,成功率（100％）高于常规法（80％）,但组间比较差异无统计学意义（P＞0.05）.结论 改良阴茎海绵体穿刺法具有穿刺海绵体成功率高的优点,在研究勃起功能的动物实验中应用.Objective To investigate the modified method of intracavernous cannulation in monitoring intracavernous pressure （ICP） of rats.Methods The present study was conducted from October to December 2014.Twenty male Sprague-Dawley rats were randomly divided into 2 groups,the conventional group （cannulated in the crus of the penis） and the modified group （cannulated in the corporal body of the penis）.The erectile function of the rats was assessed by measuring ICP/MAP （mean arterial pressure） ratio.The electrical-stimulation （ES） parameters were 5 V,15 Hz,5 ms,and 60 s for each stimulation.Results The differences in the basic ICP （14.9 ± 2.7 versus 15.5 ± 3.1 mmHg）,ES-ICP （87.6 ± 7.5 versus 85.0 ± 6.2 mmHg）,and ICP/MAP （71.3% ± 6.6% versus 72.5% ± 6.3%） were not significant between the 2 groups （P 〉0.05 for all）.Compared with the conventional group,the modified group could accurately cannulate the corpus cavernosum under direct vision,with an improved successful rate （100% versus 80%）,but there was no significant difference （P 〉 0.05） in intracavernous pressure measurement.Conclusion The modified method of cannulating in the corpus cavernosum could have the advantage of higher successful rate,suggesting a feasible way for basic research of erectile dysfunction in rats.

关 键 词：勃起功能障碍 阴茎海绵体穿刺 改良 阴茎海绵体内压 平均动脉压 

分 类 号：R698[医药卫生—泌尿科学]